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Por favor, use este identificador para citar o enlazar este ítem: http://uvadoc.uva.es/handle/10324/32538
Título: Single-cell phenotypic characterization of human pituitary GHomas and non-functioning adenomas based on hormone content and calcium responses to hypothalamic releasing hormones
Autor: Senovilla, Laura
Núñez Llorente, Lucía
Campos, José María de
Luis Román, Daniel Antonio de
Romero Bobillo, Enrique
García-Sancho, Javier
Villalobos, Carlos
Año del Documento: 2015
Editorial: Frontiers Media
Descripción: Producción Científica
Documento Fuente: Frontiers in Oncology, 2015, 5:124
Resumen: Human pituitary tumors are generally benign adenomas causing considerable morbidity due to excess hormone secretion, hypopituitarism, and other tumor mass effects. Pituitary tumors are highly heterogeneous and difficult to type, often containing mixed cell phenotypes. We have used calcium imaging followed by multiple immunocytochemistry to type growth hormone secreting (GHomas) and non-functioning pituitary adenomas (NFPAs). Individual cells were typed for stored hormones and calcium responses to classic hypothalamic releasing hormones (HRHs). We found that GHomas contained growth hormone cells either lacking responses to HRHs or responding to all four HRHs. However, most GHoma cells were polyhormonal cells responsive to both thyrotropin-releasing hormone (TRH) and GH-releasing hormone. NFPAs were also highly heterogeneous. Some of them contained ACTH cells lacking responses to HRHs or polyhormonal gonadotropes responsive to LHRH and TRH. However, most NFPAs were made of cells storing no hormone and responded only to TRH. These results may provide new insights on the ontogeny of GHomas and NFPAs.
Palabras Clave: Hormonas liberadoras hipotalámicas
Hypothalamic releasing hormones
ISSN: 2234-943X
Revisión por Pares: SI
DOI: https://doi.org/10.3389/fonc.2015.00124
Patrocinador: Ministerio de Economía, Industria y Competitividad (Project BFU2012-37146)
Instituto de Salud CarlosIII (FIS03/1231)
Version del Editor: https://www.frontiersin.org/articles/10.3389/fonc.2015.00124/full#h7
Idioma: eng
URI: http://uvadoc.uva.es/handle/10324/32538
Derechos: info:eu-repo/semantics/openAccess
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