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dc.contributor.authorFlora, Tatjana
dc.contributor.authorGonzález de Torre, Israel 
dc.contributor.authorAlonso Rodrigo, Matilde 
dc.contributor.authorRodríguez Cabello, José Carlos 
dc.date.accessioned2019-04-03T11:03:44Z
dc.date.available2019-04-03T11:03:44Z
dc.date.issued2019
dc.identifier.citationJournal of Materials Science: Materials in Medicine, 2019, vol. 30, n. 30. 12 p.es
dc.identifier.issn0957-4530es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/35327
dc.descriptionProducción Científicaes
dc.description.abstractThe development of new capillary networks in engineered constructs is essential for their survival and their integration with the host tissue. It has recently been demonstrated that ELR-based hydrogels encoding different bioactivities are able to modulate their interaction with the host after injection or implantation, as indicated by an increase in cell adhesion and the ability to trigger vascularization processes. Accordingly, the aim of this study was to increase their angiogenic ability both in vitro and in vivo using a small VEGF mimetic peptide named QK, which was tethered chemically to ELR-based hydrogels containing cell-adhesion sequences in their backbone, such as REDV and RGD, as well as a proteolytic site (VGVAPG). In vitro studies were performed using a co-culture of endothelial and fibroblast cells encapsulated into the ELR-based hydrogels in order to determine cell proliferation after 21 days of culture, as well as the number of cell-cell interactions. It was found that although the presence of this peptide does not influence the morphological and rheological properties of these hydrogels, it has an effect on cell behaviour, inducing an increase in cell proliferation and the formation of endothelial cell clusters. In vivo studies demonstrate that the QK peptide enhances the formation of prominent functional capillaries at three weeks post-injection, as confirmed by H&E staining and CD31 immunohistochemistry. The newly formed functional microvasculature ensures perfusion and connection with surrounding tissues. These results show that ELR-QK hydrogels increase capillary network formation and are therefore attractive candidates for application in tissue regeneration, for example for the treatment of cardiovascular diseases such as myocardial infarction or ischemia.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherSpringer Linkes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.classificationPéptido QKes
dc.subject.classificationQK peptidees
dc.subject.classificationAngiogénesises
dc.subject.classificationAngiogenesises
dc.subject.classificationHidrogeles de elastámero recombinantees
dc.subject.classificationElastin-like recombinamer hydrogelses
dc.titleTethering QK peptide to enhance angiogenesis in elastin-like recombinamer (ELR) hydrogelses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doihttps://doi.org/10.1007/s10856-019-6232-zes
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10856-019-6232-zes
dc.peerreviewedSIes
dc.description.projectEuropean Commission (NMP-2014-646075, PITN-GA-2012-317306)es
dc.description.projectMinisterio de Economía, Industria y Competitividad (Projects PCIN-2015-010, MAT2015- 68901-R, MAT2016-78903-R)es
dc.description.projectJunta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. VA015U16)es
dc.description.projectCentro en Red de Medicina Regenerativa y Terapia Celular de Castilla y Leónes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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