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dc.contributor.authorPérez Fernández, Rebeca 
dc.contributor.authorCasal Martínez, Jesús
dc.contributor.authorMuñoz Torre, Raúl 
dc.contributor.authorLebrero Fernández, Raquel 
dc.date.accessioned2019-07-08T18:48:08Z
dc.date.available2019-07-08T18:48:08Z
dc.date.issued2019
dc.identifier.citationScience of The Total Environment, Volume 688, 2019, Pages 684-690es
dc.identifier.issn0048-9697es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/36737
dc.descriptionProducción Científicaes
dc.description.abstractThe isolation of highly efficient methanotrophic communities is crucial for the optimization of methane bioconversion into products with a high market value such as polyhydroxyalkanoates (PHA). The research here presented aimed at enriching a methanotrophic consortium from two different inocula (Sphagnum peat moss (Sp) and Sphagnum and activated sludge (M)) able to accumulate PHA while efficiently oxidizing CH4. Moreover, the effect of the temperature and phosphorus limitation on the biodegradation rate of CH4 and the PHA accumulation potential was investigated. Higher CH4 degradation rates were obtained under P availability at increasing temperature (25, 30 and 37 °C). The biomass enriched from the mixed inoculum always exhibited a superior biodegradation performance regardless of the temperature (a maximum value of 84.3 ± 8.4 mg CH4 h−1 g biomass−1 was recorded at 37 °C). The results of the PHB production showed that phosphorus limitation is required to promote PHB accumulation, the highest PHB content being observed with the Sphagnum inoculum at 25 °C (13.6 ± 5.6%). The differential specialization of the microbial communities depending on the enrichment temperature supported the key role of this parameter on the results obtained. In all cases after the completion of the enrichment process and of the P limitation tests, Methylocystis, a type II methanotroph known for its ability to accumulate PHA, was the genus that became dominant (reaching percentages from 16 to 46% depending on the enrichment temperature). Thus, the results here obtained demonstrated for the first time the relevance of the temperature used for the enrichment of the methanotrophic bacteria to boost PHA production yields under P limiting condition, highlighting the importance of optimizing culture conditions to improve the cost-efficiency of bioprocesses based on using methane as the primary feedstock for the PHA industrial market.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titlePolyhydroxyalkanoates production from methane emissions in Sphagnum mosses: Assessing the effect of temperature and phosphorus limitationes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2019 Elsevieres
dc.identifier.doi10.1016/j.scitotenv.2019.06.296es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0048969719328694es
dc.identifier.publicationfirstpage684es
dc.identifier.publicationlastpage690es
dc.identifier.publicationtitleScience of The Total Environmentes
dc.identifier.publicationvolume688es
dc.peerreviewedSIes
dc.description.projectMinisterio de Economía, Industria y Competitividad - FEDER (Project CTM2015-73228-JIN)es
dc.description.projectJunta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. UIC 71 and CLU 2017-09)es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/submittedVersiones


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