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dc.contributor.authorLorenzo Hernando, Ana María 
dc.contributor.authorRuiz Vegas, Javier
dc.contributor.authorVega Alegre, María del Sol 
dc.contributor.authorBolado Rodríguez, Silvia 
dc.date.accessioned2019-09-17T07:52:43Z
dc.date.available2019-09-17T07:52:43Z
dc.date.issued2019
dc.identifier.citationBioresource Technology, 2019, vol. 273. p. 599-607es
dc.identifier.issn0960-8524es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/37973
dc.descriptionProducción Científicaes
dc.description.abstractThe influence of three variables on key parameters of the protein extraction process (an alkaline hydrolysis followed by an acidic precipitation) for biomass from innovative photo-bioreactors for pig manure treatment was evaluated. Alkaline hydrolysis provided high solubilisation values (up to 66.5% of the biomass), augmenting with increasing values of the three studied variables (NaOH concentration, temperature and time). Nevertheless, moderate total (13.2%) and protein extraction yields (16.9%) were obtained, which was attributable to protein denaturation or to the low effectivity of the precipitation method. Extracts rich in proteins (53.5%–77.9%) with suitable amino acid profiles were obtained, but significant amounts of the initial lipids (up to 44.6%) were co-extracted probably due to fatty acids saponification. These results establish the first step for future studies in enhancing cell wall disruption and protein recovery by coupling alkaline hydrolysis with other mechanical pre-treatments, while considering alternative separation and purification methods.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.classificationProtein extractiones
dc.subject.classificationExtracción de proteínases
dc.subject.classificationLipidses
dc.subject.classificationLípidoses
dc.subject.classificationpH-shiftes
dc.subject.classificationVariación de pHes
dc.subject.classificationSolubilisationes
dc.subject.classificationSolubilizaciónes
dc.subject.classificationWastewateres
dc.subject.classificationAguas residualeses
dc.titleRecovery of proteins from biomass grown in pig manure microalgae-based treatment plants by alkaline hydrolysis and acidic precipitationes
dc.typeinfo:eu-repo/semantics/articlees
dc.rights.holder© 2019 Elsevieres
dc.identifier.doihttps://doi.org/10.1016/j.biortech.2018.11.068es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0960852418316018es
dc.peerreviewedSIes
dc.description.projectJunta de Castilla y León (Projects UIC 071 / CLU 2017-09 / VA080G18)es
dc.description.projectMinisterio de Ciencia, Innovación y Universidades (Project CTQ2017-84006-C3-1-R)es
dc.description.projectEU-FEDER (Projects CLU 2017-09 / CTQ2017-84006-C3-1-R)es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.type.hasVersioninfo:eu-repo/semantics/submittedVersiones


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