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Título
Increased Intraepithelial Vα24 Invariant NKT Cells in the Celiac Duodenum
Autor
Año del Documento
2015
Editorial
MDPI
Descripción
Producción Científica
Documento Fuente
Nutrients 2015, 7, 8960–8976
Abstract
Celiac Disease (CD) is an interferon (IFN)
-mediated duodenal hypersensitivity to wheat
gluten occurring in genetically predisposed individuals. Gluten-free diet (GFD) leads to a complete
remission of the disease. V 24-restricted invariant NKT (iNKT) cells are important to maintain
immune homeostasis in the gut mucosa because of their unique capacity to rapidly produce large
quantities of both T-helper (Th)1 and Th2 cytokines upon stimulation. We studied the presence of
these cells in the CD duodenum. Duodenal biopsies were obtained from 45 untreated-CD patients
(uCD), 15 Gluten Free Diet-CD patients (GFD-CD), 44 non-inflamed non-CD controls (C-controls)
and 15 inflamed non-CD controls (I-controls). Two populations from Spain and Argentina were
recruited. Messenger RNA (mRNA) expression of V 24-J 18 (invariant TCR chain of human iNKT
cells), IFN
and intracellular transcription factor Forkhead Box P3 (Foxp3), and flow cytometry
intraepithelial lymphocyte (IEL) profile were determined. Both uCD and GFD-CD patients had
higher V 24-J 18 mRNA levels than non-CD controls (I and C-controls). The expression of
V 24-J 18 correlated with Marsh score for the severity of mucosal lesion and also with increased
mRNA IFN
levels. uCD and GFD-CD patients had decreased mRNA expression of FoxP3 but
increased expression of V 24-J 18, which revealed a CD-like molecular profile. Increased numbers
of iNKT cells were confirmed by flow cytometry within the intraepithelial lymphocyte compartment
of uCD and GFD-CD patients and correlated with V 24-J 18 mRNA expression. In conclusion, we
have found an increased number of iNKT cells in the duodenum from both uCD and GFD-CD
patients, irrespective of the mucosal status. A CD-like molecular profile, defined by an increased
mRNA expression of V 24-J 18 together with a decreased expression of FoxP3, may represent a
pro-inflammatory signature of the CD duodenum.
Materias Unesco
3206.10 Enfermedades de la Nutrición
Palabras Clave
Enfermedad celíaca
ISSN
2072-6643
Revisión por pares
SI
Patrocinador
Instituto de Salud Carlos III-FEDER (PI070244, PI10/01647)
Junta de Castilla y León (SAN673/VA22-08)
Junta de Castilla y León (SAN673/VA22-08)
Version del Editor
Propietario de los Derechos
© MDPI
Idioma
eng
Tipo de versión
info:eu-repo/semantics/publishedVersion
Derechos
openAccess
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