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    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/41116

    Título
    Increased Intraepithelial Vα24 Invariant NKT Cells in the Celiac Duodenum
    Autor
    Montalvillo Álvarez, Enrique
    Bernando, David
    Martínez Abad, Beatriz
    Allegretti, Yessica
    Fernández Salazar, Luis Ignaciountranslated
    Calvo, Carmen
    Chirdo, Fernando G.
    Garrote Adrados, José Antoniountranslated ORCID
    Arranz Sanz, Eduardountranslated ORCID
    Año del Documento
    2015
    Editorial
    MDPI
    Descripción
    Producción Científica
    Documento Fuente
    Nutrients 2015, 7, 8960–8976
    Abstract
    Celiac Disease (CD) is an interferon (IFN) -mediated duodenal hypersensitivity to wheat gluten occurring in genetically predisposed individuals. Gluten-free diet (GFD) leads to a complete remission of the disease. V 24-restricted invariant NKT (iNKT) cells are important to maintain immune homeostasis in the gut mucosa because of their unique capacity to rapidly produce large quantities of both T-helper (Th)1 and Th2 cytokines upon stimulation. We studied the presence of these cells in the CD duodenum. Duodenal biopsies were obtained from 45 untreated-CD patients (uCD), 15 Gluten Free Diet-CD patients (GFD-CD), 44 non-inflamed non-CD controls (C-controls) and 15 inflamed non-CD controls (I-controls). Two populations from Spain and Argentina were recruited. Messenger RNA (mRNA) expression of V 24-J 18 (invariant TCR chain of human iNKT cells), IFN and intracellular transcription factor Forkhead Box P3 (Foxp3), and flow cytometry intraepithelial lymphocyte (IEL) profile were determined. Both uCD and GFD-CD patients had higher V 24-J 18 mRNA levels than non-CD controls (I and C-controls). The expression of V 24-J 18 correlated with Marsh score for the severity of mucosal lesion and also with increased mRNA IFN levels. uCD and GFD-CD patients had decreased mRNA expression of FoxP3 but increased expression of V 24-J 18, which revealed a CD-like molecular profile. Increased numbers of iNKT cells were confirmed by flow cytometry within the intraepithelial lymphocyte compartment of uCD and GFD-CD patients and correlated with V 24-J 18 mRNA expression. In conclusion, we have found an increased number of iNKT cells in the duodenum from both uCD and GFD-CD patients, irrespective of the mucosal status. A CD-like molecular profile, defined by an increased mRNA expression of V 24-J 18 together with a decreased expression of FoxP3, may represent a pro-inflammatory signature of the CD duodenum.
    Materias Unesco
    3206.10 Enfermedades de la Nutrición
    Palabras Clave
    Enfermedad celíaca
    ISSN
    2072-6643
    Revisión por pares
    SI
    DOI
    10.3390/nu7115444
    Patrocinador
    Instituto de Salud Carlos III-FEDER (PI070244, PI10/01647)
    Junta de Castilla y León (SAN673/VA22-08)
    Version del Editor
    https://www.mdpi.com/2072-6643/7/11/5444
    Propietario de los Derechos
    © MDPI
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/41116
    Tipo de versión
    info:eu-repo/semantics/publishedVersion
    Derechos
    openAccess
    Collections
    • DEP55 - Artículos de revista [94]
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    Attribution-NonCommercial-NoDerivatives 4.0 InternacionalExcept where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional

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