2024-03-28T15:55:26Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/220852021-06-23T09:50:21Zcom_10324_1133com_10324_931com_10324_894col_10324_1209
2017-01-12T09:06:22Z
urn:hdl:10324/22085
Tissue reaction after intrastromal corneal ring implantation in an experimental animal model
Ibares Frías, Lucía
Gallego Muñoz, Patricia
Cantalapiedra Rodríguez, Roberto
Cruz Valsero, Maria Cruz
Mar Sardaña, Santiago
Merayo Lloves, Jesús
Martínez García, María del Carmen
Gallinas - Ensayos técnicos
Córnea - Cirugía
Producción Científica
Purpose To evaluate corneal wound healing in the hen animal
model after additive surgery with an intracorneal ring segment
(ICRS).
Methods We implanted one ICRS in each eye of 76 hens. In
control group 1 (n=22 hens), the stromal channel was prepared
but no ICRS was inserted. In control group 2 (n=2
hens), no surgery was performed. Animals were randomly
separated into groups and euthanized after clinical follow-up
of 4 and 12 hours, 1, 2, 3, and 7 days, and 1, 2, 3, 4, and
6 months. Corneas were stained with hematoxylin-eosin. Apoptosis
was measured by terminal uridine nick end-labeling
assays. Cell proliferation and myofibroblast-like differentiation
were assayed by BrdU and α-smooth muscle actin immunofluorescence
microscopy. Stromal matrix changes were
documented by electron microscopy.
Results Epithelial and stromal cell apoptosis around the
ICRS-implanted and control group 1 eyes peaked at
12 hours, but continued for 72 hours. In ICRSimplanted
eyes, epithelial and stromal proliferation was
present at 12 and 24 hours, respectively, and peaked at
7 days and 72 hours, respectively. Some proliferation in
the ICRS-implanted group continued through the 6-
month follow-up, and myofibroblast-like cells differentiated
one to three months after ICRS implantation. The
segments rotated within the stroma as the limbal inferior
angle approached the epithelium.
Conclusions Wound healing after ICRS implantation in hen
corneas was similar to that of other corneal surgical wounds in
stages. However, there were some specific features related to
the small size of the epithelial wound and the device permanently
implanted inside the cornea.
2017-01-12T09:06:22Z
2017-01-12T09:06:22Z
2015
info:eu-repo/semantics/article
Graefe's Archive for Clinical and Experimental Ophthalmology, (2015); 253:1071–1083
0721-832X
http://uvadoc.uva.es/handle/10324/22085
10.1007/s00417-015-2959-5
1071
1083
Graefe's Archive for Clinical and Experimental Ophthalmology
253
eng
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Springer Verlag