2024-03-28T16:35:01Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/364222021-06-24T07:27:41Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
2019-06-25T10:41:24Z
urn:hdl:10324/36422
Use of proteolytic sequences with different cleavage kinetics as a way to generate hydrogels with preprogrammed cell-infiltration patterns imparted over their given 3D spatial structure
Flora, Tatjana
González de Torre, Israel
Alonso Rodrigo, Matilde
Rodríguez Cabello, José Carlos
Biomaterials
Biomateriales
Angiogenesis
Angiogénesis
Elastin-like recombinamers
Recombinantes tipo elastina
Producción Científica
Control over biodegradation processes is crucial to generate advanced functional structures with a more interactive and efficient role for biomedical applications. Herein, a simple, high-throughput approach is developed based on a 3D-structured system that allows a preprogramed spatial-temporal control over cell infiltration and biodegradation. The 3D-structured system is based on elastin-like recombinamers (ELRs) characterized by differences in the kinetics of their peptide cleavage and consists of a three-layer hydrogel disk comprising an internal layer containing a rapidly degrading component, with the external layers containing a slow-degrading ELR. This structure is intended to invert the conventional pattern of cell infiltration, which goes from the outside to the inside of the implant, to allow an anti-natural process in which infiltration takes place first in the internal layer and later progresses to the outer layers. Time-course in vivo studies proved this hypothesis, i.e. that it is possible to drive the infiltration of cells over time in a given 3D-structured implant in a controlled and predesigned way that is able to overcome the natural tendency of conventional cell infiltration. The results obtained herein open up the possibility of applying this concept to more complex systems with multiple biological functions.
2019-06-25T10:41:24Z
2019-06-25T10:41:24Z
2019
info:eu-repo/semantics/article
Biofabrication, 2019, vol. 11, n. 3. 16 p.
1758-5090
http://uvadoc.uva.es/handle/10324/36422
https://doi.org/10.1088/1758-5090/ab10a5
1758-5090
eng
https://iopscience.iop.org/article/10.1088/1758-5090/ab10a5
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2019 IOP Publishing
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