2024-03-28T15:18:19Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/101172021-06-23T09:49:27Zcom_10324_1133com_10324_931com_10324_894col_10324_1209
CD229 (Ly9) lymphocyte cell surface receptor Interacts homophilically through Its N-Terminal domain and relocalizes to the immunological synapse
Romero, Xavier
Zapater, Nuria
Calvo, María
Kalko, Susana G.
Fuente García, Miguel Ángel de la
Tovar, Victoria
Ockeloen, Charlotte
Pizcueta, Pilar
Engel, Pablo
Inmunología
Producción Científica
CD229 is a member of the CD150 family of the Ig superfamily expressed on T and B cells. Receptors of this family regulate
cytokine production and cytotoxicity of lymphocytes and NK cells. The cytoplasmic tail of CD229 binds to SAP, a protein that is
defective in X-linked lymphoproliferative syndrome. To identify the CD229 ligand, we generated a soluble Ig fusion protein
containing the two N-terminal extracellular domains of human CD229 (CD229-Ig). CD229-Ig bound to CD229-transfected cells,
whereas no binding was detected on cells expressing other CD150 family receptors, showing that CD229 binds homophilically.
Both human and mouse CD229 interacted with itself. Domain deletion mutants showed that the N-terminal Ig-domain mediates
homophilic adhesion. CD229-CD229 binding was severely compromised when the charged amino acids E27 and E29 on the
predicted B-C loop and R89 on the F-G loop of the N-terminal domain were mutated to alanine. In contrast, one mutation, R44A,
enhanced the homophilic interaction. Confocal microscopy image analysis revealed relocalization of CD229 to the contact area of
T and B cells during Ag-dependent immune synapse formation. Thus, CD229 is its own ligand and participates in the immunological
synapse.
2015-03-26
2015-03-26
2005
info:eu-repo/semantics/article
The Journal of Immunology, 2005, vol. 174, n. 11. p. 7033–7042.
0022-1767
http://uvadoc.uva.es/handle/10324/10117
10.4049/jimmunol.174.11.7033
7033
11
7042
The Journal of Immunology
174
eng
https://www.jimmunol.org/content/174/11/7033
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
American Association of Immunologists