2024-03-28T10:17:18Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/403482021-06-24T07:50:13Zcom_10324_40357com_10324_952com_10324_894com_10324_1133com_10324_931col_10324_40358col_10324_1209
Poly-l/dl-lactic acid films functionalized with collagen IV as carrier substrata for corneal epithelial stem cells
De La Mata Sampedro, Ana
Mateos Timoneda, Miguel Ángel
Nieto Miguel, Teresa
Galindo de la Rosa, Sara
López Paniagua, Marina
Planell, Josep A.
Engel, Elisabeth
Calonge, Margarita
Limbal epithelial stem cells (LESCs) are responsible for the renewal of corneal epithelium. Cultivated limbal epithelial transplantation is the current treatment of choice for restoring the loss or dysfunction of LESCs. To perform this procedure, a substratum is necessary for in vitro culturing of limbal epithelial cells and their subsequent transplantation onto the ocular surface. In this work, we evaluated poly-L/DL-lactic acid 70:30 (PLA) films functionalized with type IV collagen (col IV) as potential in vitro carrier substrata for LESCs. We first demonstrated that PLA-col IV films were biocompatible and suitable for the proliferation of human corneal epithelial cells. Subsequently, limbal epithelial cell suspensions, isolated from human limbal rings, were cultivated using culture medium that did not contain animal components. The cells adhered significantly faster to PLA-col IV films than to tissue culture plastic (TCP). The mRNA expression levels for the LESC specific markers, K15, P63α and ABCG2 were similar or greater (significantly in the case of K15) in limbal epithelial cells cultured on PLA-col IV films than limbal epithelial cells cultured on TCP. The percentage of cells expressing the corneal (K3, K12) and the LESC (P63α, ABCG2) specific markers was similar for both substrata. These results suggest that the PLA-col IV films promoted LESC attachment and helped to maintain their undifferentiated stem cell phenotype. Consequently, these substrata offer an alternative for the transplantation of limbal cells onto the ocular surface.
2020-01-24
2020-01-24
2019
info:eu-repo/semantics/article
Colloids and Surfaces B: Biointerfaces: 2019. 177: 121-129
0927-7765
http://uvadoc.uva.es/handle/10324/40348
10.1016/j.colsurfb.2019.01.054
121
129
Colloids and Surfaces B: Biointerfaces
177
eng
https://www.sciencedirect.com/science/article/pii/S0927776519300542?via%3Dihub
https://doi.org/10.1016/j.colsurfb.2019.01.054
info:eu-repo/semantics/openAccess
ELSEVIER, Radarweg 29, 1043 NX Amsterdam, The Netherlands
Elsevier