2024-03-28T22:14:37Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/218462021-06-23T11:48:38Zcom_10324_1185com_10324_931com_10324_894col_10324_1346
00925njm 22002777a 4500
dc
Inostroza Brito, Karla E.
author
Collin, Estelle
author
Siton Mendelson, Orit
author
Smith, Katherine H.
author
Monge Marcet, Amália
author
Ferreira, Daniela S.
author
Pérez Rodríguez, Raúl
author
Alonso Rodrigo, Matilde
author
Rodríguez Cabello, José Carlos
author
Reis, Rui Luís
author
Sagués, Francesc
author
Botto, Lorenzo
author
Bitton, Ronit
author
Azevedo, Helena S.
author
Mata, Álvaro
author
2015
Controlling molecular interactions between bioinspired molecules can enable the development of new materials with higher complexity and innovative properties. Here we report on a dynamic system that emerges from the conformational modification of an elastin-like protein by peptide amphiphiles and with the capacity to access, and be maintained in, non- equilibrium for substantial periods of time. The system enables the formation of a robust membrane that displays controlled assembly and disassembly capabilities, adhesion and sealing to surfaces, self-healing and the capability to undergo morphogenesis into tubular structures with high spatiotemporal control. We use advanced microscopy along with turbidity and spectroscopic measurements to investigate the mechanism of assembly and its relation to the distinctive membrane architecture and the resulting dynamic properties. Using cell-culture experiments with endothelial and adipose- derived stem cells, we demonstrate the potential of this system to generate complex bioactive scaffolds for applications such as tissue engineering.
Nature Chemistry, 2015, vol. 7, p. 897–904
ISSN: 1755-4330
http://uvadoc.uva.es/handle/10324/21846
10.1038/nchem.2349
897
904
Nature Chemistry
Vol. 7
Moléculas - Propiedades
Co-assembly, spatiotemporal control and morphogenesis of a hybrid protein–peptide system.