2024-03-29T10:56:54Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/349082022-07-28T11:50:18Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
00925njm 22002777a 4500
dc
Cipriani, Filippo
author
Krüger, Melanie
author
González de Torre, Israel
author
Quintanilla Sierra, Luis
author
Alonso Rodrigo, Matilde
author
Kock, Linda
author
Rodríguez Cabello, José Carlos
author
2018
Tissue engineering for cartilage repair requires biomaterials that show rapid gelation and adequate mechanical properties. Although the use of hydrogel is the most promising biomaterial, it often lacks in rigidity and anchorage of cells when they are surrounded by synovial fluid while they are subjected to heavy loads. We developed and produced the Silk Elastin-Like co-Recombinamer (SELR), which contains both the physical interaction from elastin motifs and from silk motifs. In the first part of this work, we set up and optimized a preannealing treatment based on the evolution of silk motifs into β-sheet structures in order to fulfill the required mechanical properties of hydrogels for cartilage repair. The new preannealed SELRs (pA(EIS)2-(I5R)6) were characterized with the combination of several experimental techniques (CD, TEM, SEM, and rheology) to provide a deep insight into the material features. Finally, the regeneration properties of the pA(EIS)2-(I5R)6 hydrogel embedded with chondrocytes were evaluated. After 4 weeks of culturing in a standardized and representative ex vivo model, the biochemical and histological analysis revealed the production of glycosaminglycans and collagen. Moreover, the immunohistochemistry showed the absence of fibro-cartilage and the presence of hyaline cartilage. Hence, we conclude that the pA(EIS)2-(I5R)6 hydrogel presents improved mechanical properties while conserving the injectability, which leads to successful regeneration of hyaline cartilage in an ex vivo model.
Biomacromolecules, 2018, 19 (11), pp 4333–4347
1525-7797
http://uvadoc.uva.es/handle/10324/34908
10.1021/acs.biomac.8b01211
Cartilage Regeneration in Preannealed Silk Elastin-Like Co-Recombinamers Injectable Hydrogel Embedded with Mature Chondrocytes in an Ex Vivo Culture Platform