2024-03-29T04:43:09Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/218372021-06-23T09:53:46Zcom_10324_1134com_10324_931com_10324_894col_10324_1214
UVaDOC
author
Calvo Rodríguez, María
author
Villalobos Jorge, Carlos
author
Núñez Llorente, Lucía
2016-12-20T08:17:21Z
2016-12-20T08:17:21Z
2015
Lossi, Laura and Merighi, Adalberto, editors. Neuronal Cell Death: Methods and Protocols, Springer, 2015, p. 73 – 85 (Methods in Molecular Biology; 1254)
978-1-4939-2152-2
http://uvadoc.uva.es/handle/10324/21837
Intracellular Ca2+ is involved in control of a large variety of cell functions including apoptosis and neuron
cell death. For example, intracellular Ca2+ overload is critical in neuron cell death induced by excitotoxicity.
Thus, single cell monitoring of intracellular Ca2+ concentration ([Ca2+]cyt) in neurons concurrently
with apoptosis and neuron cell death is widely required.
Procedures for culture and preparation of primary cultures of hippocampal rat neurons and fluorescence
imaging of cytosolic Ca2+ concentration in Fura2/AM-loaded neurons are described. We also
describe a method for apoptosis detection by immunofluorescence imaging. Finally, a simple method for
concurrent measurements of [Ca2+]cyt and apoptosis in the same neurons is described.
eng
Calcium imaging
Neuron death
Apoptosis
Hippocampal neurons
Fura2/AM
Calcium Imaging in Neuron Cell Death
info:eu-repo/semantics/bookPart
URL
https://uvadoc.uva.es/bitstream/10324/21837/1/Methods%20Mol%20Biol%202015.pdf
File
MD5
4ad15ddbead4a26c1e0a4f47f5ee9e6d
538926
application/pdf
Methods Mol Biol 2015.pdf