2024-03-28T21:15:16Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/365772021-06-24T07:27:45Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
Gala Lopez, Boris L.
Neiman, Daniel
Kin, Tatsuya
O’Gorman, Doug
Pepper, Andrew R.
Malcolm, Andrew J.
Pianzin, Sheina
Senior, Peter A.
Campbell, Patricia
Glaser, Benjamin
Dor, Yuval
Shemer, Ruth
Shapiro, A.M. James
2019-07-02T13:20:16Z
2019-07-02T13:20:16Z
2018
Transplantation, June 2018, 102. 978-985
0041-1337
http://uvadoc.uva.es/handle/10324/36577
10.1097/TP.0000000000002083
978
6
985
Transplantation
102
Background: Optimizing engraftment and early survival after clinical islet transplantation is critical to long-term function, but there are no reliable, quantifiable measures to assess beta cell death. Circulating cell free DNA (cfDNA) derived from beta cells has been identified as a novel biomarker to detect cell loss, and was recently validated in new-onset type 1 diabetes and in islet transplant patients.
Methods: Herein we report beta cell cfDNA measurements after allotransplantation in 37 subjects and the correlation with clinical outcomes.
Results: A distinctive peak of cfDNA was observed 1hr after transplantation in 31/37 (83.8%) of subjects. The presence and magnitude of this signal did not correlate with transplant outcome. The 1hr signal represents dead beta cells carried over into the recipient after islet isolation and culture, combined with acute cell death post infusion. Beta cell cfDNA was also detected 24hrs post-transplant (8/37 subjects, 21.6%). This signal was associated with higher 1-month insulin requirements (p=0.04), lower 1-month stimulated C-peptide levels (p=0.01) and overall worse 3-month engraftment, by insulin independence (ROC:AUC=0.70, p=0.03) and Beta 2 score (ROC:AUC=0.77, p=0.006).
Conclusions: cfDNA-based estimation of beta cell death 24hrs after islet allotransplantation correlates with clinical outcome and could predict early engraftment.
spa
info:eu-repo/semantics/openAccess
Beta cell death by cell-free DNA and outcome after clinical islet transplantation
info:eu-repo/semantics/article