2024-03-29T10:30:31Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/408202021-09-20T08:58:02Zcom_10324_1183com_10324_931com_10324_894col_10324_1396
Muñoz Adalia, Emigdio Jordán
Cañizares Ruiz, María del Carmen
Fernández Fernández, María Mercedes
Díez Casero, Julio Javier
García Pedrajas, María Dolores
2020-05-06T12:59:01Z
2020-05-06T12:59:01Z
2018
Forests, 2018, vol. 9, n. 11. 12 p.
1999-4907
http://uvadoc.uva.es/handle/10324/40820
10.3390/f9110684
Fusarium circinatum is the causal agent of pine pitch canker (PPC), one of the most
devastating forest diseases worldwide. This fungus causes severe damping-off in pine seedlings and
growth reduction, wilting and the development of cankers in pine forests and plantations. A draft of
the complete genome sequence of this phytopathogen was recently made available. This information
was used to annotate in silico the gene Fcrho1 as a putative homolog of Rho1 GTPase genes. In this
study, we generated Fcrho1 deletion mutants in two F. circinatum wildtype strains isolated from
damaged trees in northern Spain. For that, we used a modified version of the OSCAR methodology,
an approach not previously used in F. circinatum that allows the generation of deletion constructs
in a single cloning step. The conidiation and spore germination of the resulting deletion mutants
were not affected, neither the hyphal morphology. However, the mutant strains showed significantly
reduced growth in vitro and more foamy macroscopic hyphal morphology than their corresponding
ectopic and wildtype strains. Finally, an in vivo virulence assay showed that the reduced in vitro
growth rate characteristic to the deletion mutants does not impact their pathogenicity.
eng
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2018 MDPI
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
The Fusarium Circinatum gene Fcrho1, encoding a putative Rho1 GTPase, is involved in vegetative growth but dispensable for pathogenic development
info:eu-repo/semantics/article