2024-03-29T09:23:37Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/43302021-09-13T09:17:18Zcom_10324_1181com_10324_931com_10324_894col_10324_1387
León, Alberto José
Garrote Adrados, José Antonio
Arranz Sanz, Eduardo
2014-02-14T14:09:16Z
2014-02-14T14:09:16Z
2006
Medicina Clínica, vol.127 n. 4 p.145-152
http://uvadoc.uva.es/handle/10324/4330
145
4
152
Medicina clínica
127
Dietary gluten induces an early response in the intestine of coeliac disease
patients (CD), within a few hours, and this is driven by high levels of proinflammatory
cytokines, including IFNg and IL-15, as has been thoroughly
shown by gluten stimulation of biopsy explants. Our aim was to identify the
immune mediators involved in the long-standing inflammation in untreated
CD patients at diagnosis. mRNA and protein levels of TNFa, IL-12(p35),
IL-12(p40), IL-15, IL-18 and IL-23(p19) were quantified in biopsies from
active CD patients, CD patients on a gluten-free diet (GFD), healthy controls,
and patients with non-CD inflammation and mild histological changes in the
intestine. Biopsies from CD patients on a GFD were also stimulated in vitro
with gliadin, and protein expression of IL-15 and IL-18 was analysed. Levels of
IL-12 and IL-23 mRNA are nearly absent, and TNFa levels remain unchanged
among different groups. Both the active and inactive forms of IL-18 protein
have been found in all samples from active CD, and protein expression was
only localized within the crypts. Levels of IL-15 mRNA remain unchanged,
and protein expression, localized within the lamina propria, is found in a
small number of samples. In vitro stimulation with gluten induces the expression
of IL-15 and IL-18. In active CD, the early response following gluten
intake characterized by high IFNg levels is driven by IL-18, and probably
IL-15, and this alternates with periods of long-standing inflammation with
moderate IFNg levels, maintained by IL-18 alone.
spa
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Enfermedad inflamatoria intestinal
Citocinas
Citocinas en la patogenia de la enfermedad inflamatoria intestinal
info:eu-repo/semantics/article