2024-03-29T09:57:42Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/353312021-11-08T09:17:14Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
Tuning the stiffness of surfaces by assembling genetically engineered polypeptides with tailored amino acid sequence
Costa, Rui R.
González Pérez, Miguel
Herrero Gutiérrez, Marcos
Pires, Ricardo A.
Alonso Rodrigo, Matilde
Rodríguez Cabello, José Carlos
Reis, Rui Luís
Pashkuleva, Iva
Modificación de superficie
Surface modification
Polipéptidos
Polypeptides
Producción Científica
We introduce elastin-like recombinamers (ELRs) as polypeptides with precise amino acid
positioning to generate polypeptide coatings with tunable rigidity. Two ELRs are used: V84-
ELR, a hydrophobic monoblock, and EI-ELR, an amphiphilic diblock. Both were modified with
the amine-reactive tetrakis (hydroxymethyl) phosphonium chloride compound. We evaluated the affinity, the conformation, and the dissipative behavior of ELRs assembled on alkanethiol selfassembled coatings by quartz crystal microbalance with dissipation monitoring, multi-parametric surface plasmon resonance, and atomic force microscopy. The thickness of the polypeptide coatings showcase the preferential affinity of ELRs to NH2 and CH3 terminated surfaces. We demonstrate that V84-ELR strongly bonded to the substrate and reorganizes into an extended and more hydrated layer as the adsorbed amount increases, whereas EI-ELR has a less dissipative behavior. The results suggest that ELR adsorption depends on the amino acid sequence and the substrate chemistry, ultimately influencing the stiffness of the polypeptide coatings.
2019-07-03
2019-07-03
Fundação para a Ciência e Tecnologia (grants SFRH/BPD/95446/2013 to R.R.C. and IF/00032/2013 to I.P.)
Comisión Europea: H2020 programme-projects ELASTISLET (NMP-2014-646075), CHEM2NATURE (TWINN-2015-692333), FORECAST (WIDESPREAD-2014-2-668983), THE DISCOVERIES CTR (WIDESPREAD-01-2016-2017739572)
Ministerio de Economía, Industria y Competitividad (Project MAT2015-68901-R, MAT2016-78903-R)
Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. VA015U16)
Ministerio de Educación, Cultura y Deporte (Proyect FPU15-00448)
2019-04-04T09:54:19Z
2018
info:eu-repo/semantics/article
https://doi.org/10.1021/acs.biomac.8b00723
Biomacromolecules, 2018, Vol. 19, n. 8. p. 3401-3411
1525-7797
http://uvadoc.uva.es/handle/10324/35331
eng
https://pubs.acs.org/doi/10.1021/acs.biomac.8b00723
info:eu-repo/grantAgreement/EC/H2020/646075
info:eu-repo/grantAgreement/EC/H2020/692333
info:eu-repo/grantAgreement/EC/H2020/668983
info:eu-repo/grantAgreement/EC/H2020/739572
info:eu-repo/semantics/openAccess
© 2019 American Chemical Society
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