2024-03-28T11:11:23Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/353302021-06-24T07:27:37Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers
Flora, Tatjana
González de Torre, Israel
Quintanilla Sierra, Luis
Alonso Rodrigo, Matilde
Rodríguez Cabello, José Carlos
A simple system for cell selectivity and spatially controlled cell adhesion has been developed using model gold surfaces grafted with a combination of two ELRs containing into their backbone cell-adhesion domains such as RGD and REDV. Grafting onto gold was achieved via redox reaction through thiol groups present in amino terminal cysteine tails of the ELRs. The correlation among contact angle, SEM micrographs, AFM, XPS and QCM-D have been carried out.
After in-depth adhesion studies, a mixture of 75% ELR-REDV and 25% ELR-RGD was found to exhibit high selectivity for endothelial cells, promoting strong adhesion thereof.
Consequently, certain areas of gold surfaces (strips) were cleaned by laser ablation and functionalized with the mixture 75% ELR-REDV - 25% ELR-RGD leading to a spatial segregation of the co-culture made of HUVEC and HFF1 cells. This platform therefore exhibits selective spatial control over cell adhesion associated with the bioactive epitopes (RGD and REDV) contained in the ELR sequence, since each functionalized surface (including strips) have similar topographic, hydrophobic and mechanical properties.
2018
info:eu-repo/semantics/article
European Polymer Journal, 2018, vol. 106. p. 19-29
0014-3057
http://uvadoc.uva.es/handle/10324/35330
https://doi.org/10.1016/j.eurpolymj.2018.06.026
eng
https://www.sciencedirect.com/science/article/pii/S0014305717322061
info:eu-repo/semantics/openAccess
© 2019 Elsevier
Elsevier