2024-03-29T16:03:02Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/379732021-06-24T07:49:03Zcom_10324_35294com_10324_952com_10324_894com_10324_1173com_10324_931col_10324_35295col_10324_1371
Recovery of proteins from biomass grown in pig manure microalgae-based treatment plants by alkaline hydrolysis and acidic precipitation
Lorenzo Hernando, Ana María
Ruiz Vegas, Javier
Vega Alegre, María del Sol
Bolado Rodríguez, Silvia
The influence of three variables on key parameters of the protein extraction process (an alkaline hydrolysis followed by an acidic precipitation) for biomass from innovative photo-bioreactors for pig manure treatment was evaluated. Alkaline hydrolysis provided high solubilisation values (up to 66.5% of the biomass), augmenting with increasing values of the three studied variables (NaOH concentration, temperature and time). Nevertheless, moderate total (13.2%) and protein extraction yields (16.9%) were obtained, which was attributable to protein denaturation or to the low effectivity of the precipitation method. Extracts rich in proteins (53.5%–77.9%) with suitable amino acid profiles were obtained, but significant amounts of the initial lipids (up to 44.6%) were co-extracted probably due to fatty acids saponification. These results establish the first step for future studies in enhancing cell wall disruption and protein recovery by coupling alkaline hydrolysis with other mechanical pre-treatments, while considering alternative separation and purification methods.
2019-09-17T07:52:43Z
2019-09-17T07:52:43Z
2019-09-17T07:52:43Z
2019
info:eu-repo/semantics/article
Bioresource Technology, 2019, vol. 273. p. 599-607
0960-8524
http://uvadoc.uva.es/handle/10324/37973
https://doi.org/10.1016/j.biortech.2018.11.068
eng
https://www.sciencedirect.com/science/article/pii/S0960852418316018
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2019 Elsevier
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Elsevier