2024-03-29T07:46:06Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/258142021-11-15T13:32:01Zcom_10324_38col_10324_852
Determination of homogentisic acid using HPLC with amperometric detection at carbon paste electrodes
Lozano Alonso, María
Vega Alegre, María del Sol
Dejmková, Hana
Universidad de Valladolid. Facultad de Ciencias
Homogentisic acid is an intermediate of the metabolic breakdown of tyrosine and phenylalanine; its accumulation in body fluids suggests the break in the metabolic pathway of these compounds and related health-threatening complications. In our work, a determination method for this compound was developed using HPLC with amperometric detection on glassy carbon paste electrode. Optimum conditions were found, considering both electrode response and chromatographic parameters. Separation was performed on Kromasil 100 C18, 250 x 4.6 mm (5 μm) column using gradient elution; mobile phase consisted of acidic buffer and methanol, with methanol content changing from 5% to 30% in 10 min. Repeatability of the measurements and linearity of the electrode response were confirmed. Applicability of the method was proved by the determination in spiked urine samples.
2017-09-21T08:27:29Z
2017-09-21T08:27:29Z
2017
info:eu-repo/semantics/bachelorThesis
http://uvadoc.uva.es/handle/10324/25814
eng
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International