2024-03-28T22:12:57Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/41492021-09-13T09:28:35Zcom_10324_1181com_10324_931com_10324_894col_10324_1387
High Levels of Proinflammatory Cytokines, but not markers of tissue injury in unaffected intestinal areas from patients with IBD
León, Alberto José
Gómez, Emma
Garrote Adrados, José Antonio
Bernardo Ordiz, David
Barrera, Asterio
Marcos, José Luis
Fernández Salazar, Luis Ignacio
Velayos Jiménez, Benito
Blanco Quirós, Alfredo
Arranz Sanz, Eduardo
Intestinos - Enfermedades
Enfermedad de Chrom
Producción Científica
Intestinal alterations in IBD are triggered and maintained by an overexpression of proinflammatory cytokines. Additionally,
increased immune activation has been found in the adjacent intestinal areas without displaying any apparent histological
alterations, however, the regulatory environment is not well established. Biopsy specimens from patients with ulcerative colitis
(UC) and Crohn’s disease (CD), from both affected and unaffected areas, and also from a group of colonic biopsies from healthy
controls, were included in our study. Cytokines and markers of mucosal damage were analyzed by real-time PCR, and some of the
results confirmed by western-blot and ELISA. Levels of IFNγ, TNFα, IL-6, IL-15, IL-18, and IL-23 were increased (above healthy
controls) in both affected and unaffected areas from IBD. IL-1β, IL-6, IL-12, and IL-27 were higher in affected areas compared to
unaffected ones in UC but not CD. In general, a correlation was observed between mRNA levels of these cytokines and both iNOS
and Granzyme B. SOCS-2 and SOCS-3 were also increased in the affected areas. In conclusion, the unaffected areas from IBD show
increased levels of a restricted set of cytokines that may exert immune activating roles in these areas without being able to trigger
tissue damage.
2014-01-07T09:03:00Z
2014-01-07T09:03:00Z
2009
info:eu-repo/semantics/article
Mediators of inflammation, June 2009 p. 1-10
0962-9351
http://uvadoc.uva.es/handle/10324/4149
10.1155/2009/580450
1
10
Mediators of Inflammation
2009
eng
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Hindawi Publishing Corporation
Attribution-NonCommercial-NoDerivatives 4.0 International
Hindawi Publishing Corporation
SI