2024-03-28T10:21:35Zhttp://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/60922021-06-23T09:51:41Zcom_10324_1134com_10324_931com_10324_894col_10324_1213
Ruiz, Mario
dbd3ec08-dd1e-4e18-8ab3-2c1217b94e07
500
Wicker-Thomas, Claude
f6a44713-fef1-48e7-a077-9406abcde5b7
500
Sánchez Romero, Diego
e455b9394a603299
500
0000-0001-6296-6773
Ganfornina Álvarez, María Dolores
5a595ce54711443c
500
0000-0002-8567-4826
2014-09-19T11:49:57Z
2014-09-19T11:49:57Z
2012
Insect Biochemistry and Molecular Biology, 2012, vol. 42, p. 776-789
0965-1748
http://uvadoc.uva.es/handle/10324/6092
10.1016/j.ibmb.2012.07.005
776
789
Insect Biochemistry and Molecular Biology
42
Producción Científica
Lazarillo (Laz) is a glycosyl-phosphatidylinositol (GPI)-linked glycoprotein first characterized in the
developing nervous system of the grasshopper Schistocerca americana. It belongs to the Lipocalins,
a functionally diverse family of mostly secreted proteins. In this work we test whether the protective
capacity known for Laz homologs in flies and vertebrates (NLaz, GLaz and ApoD) is evolutionarily
conserved in grasshopper Laz, and can be exerted from the plasma membrane in a cell-autonomous
manner. First we demonstrate that extracellular forms of Laz have autocrine and paracrine protecting
effects for oxidative stress-challenged Drosophila S2 cells. Then we assay the effects of overexpressing
GPI-linked Laz in adult Drosophila and whether it rescues both known and novel phenotypes of NLaz null
mutants. Local effects of GPI-linked Laz inside and outside the nervous system promote survival upon
different stress forms, and extend lifespan and healthspan of the flies in a cell-type dependent manner.
Outside the nervous system, expression in fat body cells but not in hemocytes results in protection.
Within the nervous system, glial cell expression is more effective than neuronal expression. Laz actions
are sexually dimorphic in some expression domains. Fat storage promotion and not modifications in
hydrocarbon profiles or quantities explain the starvationedesiccation resistance caused by Laz overexpression.
This effect is exerted when Laz is expressed ubiquitously or in dopaminergic cells, but not in
hemocytes. Grasshopper Laz functionally restores the loss of NLaz, rescuing stress-sensitivity as well as
premature accumulation of aging-related damage, monitored by advanced glycation end products
(AGEs). However Laz does not rescue NLaz courtship behavioral defects. Finally, the presence of two new
Lipocalins with predicted GPI-anchors in mosquitoes shows that the functional advantages of
GPI-linkage have been commonly exploited by Lipocalins in the arthropodan lineage.
application/pdf
eng
Elsevier
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Lipocainas
Células gliales
Insectos
Grasshopper Lazarillo, a GPI-anchored Lipocalin, increases Drosophila longevity and stress resistance, and functionally replaces its secreted homolog NLaz
info:eu-repo/semantics/article
SI
ORIGINAL
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