RT info:eu-repo/semantics/article
T1 Biocompatible ELR-Based Polyplexes Coated with MUC1 Specific Aptamers and Targeted for Breast Cancer Gene Therapy
A1 Piña, María Jesús
A1 Girotti, Alessandra
A1 Santos García, María Mercedes
A1 Rodríguez Cabello, José Carlos
A1 Arias Vallejo, Francisco Javier
K1 Cáncer-Terapias
AB The search for new and biocompatible materials with high potential for improvement is a challenge in gene delivery applications. A cell type specific vector made of elastin- like recombinamer (ELR) and aptamers has been specifically designed for the intracellular delivery of therapeutic material for breast cancer therapy. A lysine-enriched ELR was constructed and complexed with plasmid DNA to give positively charged and stable polyplexes. Physical character- ization of these polyplexes showed a particle size of around 140 nm  and a  zeta  potential  of  approximately  +40  mV.  Theincorporation of MUC1-specific aptamers into the polyplexes resulted in a slight decrease in zeta potential but increased cell transfection specificity for MCF-7 breast cancer cells with respect to a MUC1-negative tumor line. After showing the transfection ability of this aptamer-ELR vector which is facilitated mainly by macropinocytosis uptake, we demonstrated its application for suicide gene therapy using a plasmid containing the gene of the toxin PAP-S. The strategy developed in this work about using ELR as polymeric vector and aptamers as supplier of specificity to deliver therapeutic material into MUC1-positive breast cancer cells shows promising potential and continues paving the way for ELRs in the biomedical field.
PB American Chemical Society
SN 1543-8384
YR 2016
FD 2016
LK http://uvadoc.uva.es/handle/10324/21697
UL http://uvadoc.uva.es/handle/10324/21697
LA eng
NO Molecular Pharmaceutics, American Chemical, 2016, vol. 7; 13; (3) p. 795-808
NO Producción Científica
DS UVaDOC
RD 23-abr-2024