RT info:eu-repo/semantics/bookPart
T1 Calcium Imaging in Neuron Cell Death
A1 Calvo Rodríguez, María
A1 Villalobos Jorge, Carlos
A1 Núñez Llorente, Lucía
K1 Calcium imaging
K1 Neuron death
K1 Apoptosis
K1 Hippocampal neurons
K1 Fura2/AM
AB Intracellular Ca2+ is involved in control of a large variety of cell functions including apoptosis and neuroncell death. For example, intracellular Ca2+ overload is critical in neuron cell death induced by excitotoxicity.Thus, single cell monitoring of intracellular Ca2+ concentration ([Ca2+]cyt) in neurons concurrentlywith apoptosis and neuron cell death is widely required.Procedures for culture and preparation of primary cultures of hippocampal rat neurons and fluorescenceimaging of cytosolic Ca2+ concentration in Fura2/AM-loaded neurons are described. We alsodescribe a method for apoptosis detection by immunofluorescence imaging. Finally, a simple method forconcurrent measurements of [Ca2+]cyt and apoptosis in the same neurons is described.
PB Springer Science+Business Media New York
SN 978-1-4939-2152-2
YR 2015
FD 2015
LK http://uvadoc.uva.es/handle/10324/21837
UL http://uvadoc.uva.es/handle/10324/21837
LA eng
NO Lossi, Laura and Merighi, Adalberto, editors. Neuronal Cell Death: Methods and Protocols, Springer, 2015, p. 73 – 85 (Methods in Molecular Biology;  1254)
NO Producción Científica
DS UVaDOC
RD 09-may-2024