RT info:eu-repo/semantics/article
T1 Recombinant DNA technology and click chemistry: a powerful combination for generating a hybrid elastin-like-statherin hydrogel to control calcium phosphate mineralization
A1 Hamed Misbah, Mohamed
A1 Santos García, María Mercedes
A1 Quintanilla Sierra, Luis
A1 Günter, Christina
A1 Alonso Rodrigo, Matilde
A1 Taubert, Andreas
A1 Rodríguez Cabello, José Carlos
K1 Calcio
K1 Mineralización
AB Understanding the mechanisms responsible for generating different phases and morphologies of calcium phosphate by elastin-like recombinamers is supreme for bioengineering of advanced multifunctional materials. The generation of such multifunctional hybrid materials depends on the properties of their counterparts and the way in which they are assembled. The success of this assembly depends on the different approaches used, such as recombinant DNA technology and click chemistry. In the present work, an elastin-like recombinamer bearing lysine amino acids distributed along the recombinamer chain has been cross-linked via Huisgen [2 + 3] cycloaddition. The recombinamer contains the SNA15 peptide domains inspired by salivary statherin, a peptide epitope known to specifically bind to and nucleate calcium phosphate. The benefit of using click chemistry is that the hybrid elastin-like-statherin recombinamers cross-link without losing their fibrillar structure. Mineralization of the resulting hybrid elastin-like-statherin recombinamer hydrogels with calcium phosphate is described. Thus, two different hydroxyapatite morphologies (cauliflower- and plate-like) have been formed. Overall, this study shows that crosslinking elastin-like recombinamers leads to interesting matrix materials for the generation of calcium phosphate composites with potential applications as biomaterials.
PB Beilstein-Institut
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/24427
UL http://uvadoc.uva.es/handle/10324/24427
LA eng
NO Beilstein Journal of Nanotechnology, 2017, 8, pg. 772–783
NO Producción Científica
DS UVaDOC
RD 24-abr-2024