RT info:eu-repo/semantics/article
T1 Coacervation of Elastin-Like Recombinamer Microgels
A1 Smriti Singh
A1 Fechete, Radu
A1 Rodríguez Cabello, José Carlos
A1 Möller, Martin
K1 Microgeles
AB The investigation of the coacervation (self-aggregation) behavior of biomicrogels which can potentially be used as drug carriers is an important topic, because self-aggregation can not only cause loss of activity, but also toxicity and immunogenicity. To study this effect microgels from elastin-like recombinamer are synthesized using miniemulsion technique. The existence of coacervation for such microgels, at different concentrations and temperatures, is studied and proved by cryo-field emission scanning clectron microscopy (cryo-FESEM), cryo-transmission electron microscopy (cryo-TEM), and by a novel 1H high-resolution magic angle sample spinning (HRMAS), nuclear magnetic resonance (NMR) spectroscopy, and relaxometry methods. The findings by 1H HRMAS NMR spectroscopy and relaxometry show simultaneous processes of volume phase temperature transition and coacervation with different sensitivity for hydrophobic and hydrophilic amino acid side-chains in the microgel. The coacervation process is more evidential by the behavior of glycine α-CH2, 1H NMR peak as compared to the proline β-CH2.
PB Wiley
YR 2016
FD 2016
LK http://uvadoc.uva.es/handle/10324/24805
UL http://uvadoc.uva.es/handle/10324/24805
LA eng
NO Macromolecular Rapid Communications, 2016, 37, Issue 2, pg.181–186
NO Producción Científica
DS UVaDOC
RD 26-abr-2024