RT info:eu-repo/semantics/bookPart T1 A Microplate-Based Bioluminescence Assay of Mitochondrial Calcium Uptake A1 Alonso Alonso, María Teresa A1 Navas Navarro, Paloma A1 García-Sancho Martín, Francisco Javier K1 Mitocondrias K1 Calcio K1 Mitochondrial Calcium AB Mitochondrial Ca2+ homeostasis is crucial for regulating vital functions such as respiration or apoptosis. Targeted aequorins are excellent probes to measure subcellular Ca2+. Ca2+ concentration in mitochondria ([Ca2+]M) is low at rest (about 10−7 M) and can increase to the micromolar or even approach the millimolar range, upon cell activation. Here we describe a new quantitative luminescent protocol to directly measure mitochondrial Ca2+ uptake, optimized for high throughput. The sensitivity of the method allows detection of changes in either the capacity or the affinity of mitochondrial Ca2+ transport. PB Humana Press SN 978-1-4939-6824-4 YR 2017 FD 2017 LK http://uvadoc.uva.es/handle/10324/32548 UL http://uvadoc.uva.es/handle/10324/32548 LA eng NO Mokranjac Dejana, Perocchi Fabiana (eds). Mitochondria: Practical Protocols. Humana Press, New York, NY, 2017. Methods in Molecular Biology book series (MIMB, 1567) NO Producción Científica DS UVaDOC RD 19-sep-2024