RT info:eu-repo/semantics/article
T1 Production of bioactive hepcidin by recombinant DNA tagging with an elastin-like recombinamer
A1 Da Costa, André
A1 Pereira, A.
A1 Gomes, A
A1 Rodríguez Cabello, José Carlos
A1 Casal, M.
A1 Machado, Raúl
K1 Hepcidina bioactiva
K1 Bioactive hepcidin
K1 Péptido antimicrobiano
K1 Antimicrobial peptides
K1 Recombinantes tipo elastina
K1 Elastin-like recombinamer
K1 Actividad antimicrobiana
K1 Antimicrobial activity
AB With the lack of new chemical antibiotics and the increasing pathogen resistance tothose available, new alternatives are being explored. Antimicrobial peptides (AMPs)with a broad range of effects such as antibacterial, antifungal, and antiviral, arise as oneof the options. These can be produced by recombinant DNA technology however, thechromatographic methods used for peptide purification are costly and time consuming.Here, we describe the design, production, purification and assessment of theantibacterial activity of the human peptide Hepcidin, using an elastin-like recombinameras fusion partner. The recombinant protein Hep-A200 was produced in Escherichia coliand purified by a non-chromatographic procedure, exploiting the thermal properties ofA200 elastin-like recombinamer. The recombinant polymer Hep-A200 retainedantibacterial activity against Gram-positive and Gram-negative species.
PB Elsevier
SN 1871-6784
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/35332
UL http://uvadoc.uva.es/handle/10324/35332
LA eng
NO New Biotechnology, 2018, vol. 46. p. 45-53
NO Producción Científica
DS UVaDOC
RD 28-abr-2024