RT info:eu-repo/semantics/article
T1 Recovery of proteins from biomass grown in pig manure microalgae-based treatment plants by alkaline hydrolysis and acidic precipitation
A1 Lorenzo Hernando, Ana María
A1 Ruiz Vegas, Javier
A1 Vega Alegre, María del Sol
A1 Bolado Rodríguez, Silvia
K1 Protein extraction
K1 Extracción de proteínas
K1 Lipids
K1 Lípidos
K1 pH-shift
K1 Variación de pH
K1 Solubilisation
K1 Solubilización
K1 Wastewater
K1 Aguas residuales
AB The influence of three variables on key parameters of the protein extraction process (an alkaline hydrolysis followed by an acidic precipitation) for biomass from innovative photo-bioreactors for pig manure treatment was evaluated. Alkaline hydrolysis provided high solubilisation values (up to 66.5% of the biomass), augmenting with increasing values of the three studied variables (NaOH concentration, temperature and time). Nevertheless, moderate total (13.2%) and protein extraction yields (16.9%) were obtained, which was attributable to protein denaturation or to the low effectivity of the precipitation method. Extracts rich in proteins (53.5%–77.9%) with suitable amino acid profiles were obtained, but significant amounts of the initial lipids (up to 44.6%) were co-extracted probably due to fatty acids saponification. These results establish the first step for future studies in enhancing cell wall disruption and protein recovery by coupling alkaline hydrolysis with other mechanical pre-treatments, while considering alternative separation and purification methods.
PB Elsevier
SN 0960-8524
YR 2019
FD 2019
LK http://uvadoc.uva.es/handle/10324/37973
UL http://uvadoc.uva.es/handle/10324/37973
LA eng
NO Bioresource Technology, 2019,  vol. 273. p. 599-607
NO Producción Científica
DS UVaDOC
RD 25-abr-2024