RT info:eu-repo/semantics/article T1 The effect of temperature during culture enrichment on methanotrophic polyhydroxyalkanoate production A1 Pérez Fernández, Rebeca A1 Cantera Ruiz de Pellón, Sara A1 Bordel Velasco, Sergio A1 García Encina, Pedro Antonio A1 Muñoz Torre, Raúl K1 Bioplásticos K1 Reducción de gases de efecto invernadero K1 Bioplastics K1 Greenhouse gas abatement AB Climate change and plastic pollution are likely the most relevant environmental problems of the 21st Century. Thus, one of the most promising solutions to remedy both environmental problems simultaneously is the bioconversion of greenhouse gases, such as methane (CH4), into bioplastics (PHAs). However, the optimization of this bioconversion platform is still required to turn CH4 biotransformation into a cost-effective and cost-competitive process. In this context, the research presented here aimed at elucidating the best temperature culture conditions to enhance both PHA accumulation and methane degradation. Six different enrichments were carried out at 25, 30 and 37 °C using different inocula and methane as the only energy and carbon source. CH4 biodegradation rates, specific growth rates, PHA accumulations and the community structure were characterized. Higher temperatures (30 and 37 °C) increased the PHAs accumulation up to 30% regardless of the inoculum. Moreover, Methylocystis became the dominant genus (∼30% of the total population) regardless of the temperature and inoculum used. This research demonstrated for the first time the fundamental role of temperature in increasing both the accumulation of PHAs and methane abatement during the enrichment of PHA cell-factories from methane, thus enhancing the cost-effectiveness of the process. PB Elsevier SN 0964-8305 YR 2019 FD 2019 LK http://uvadoc.uva.es/handle/10324/38023 UL http://uvadoc.uva.es/handle/10324/38023 LA eng NO International Biodeterioration & Biodegradation Volume 140, 2019, Pages 144-151 NO Producción Científica DS UVaDOC RD 20-sep-2024