RT info:eu-repo/semantics/article
T1 Genome scale metabolic modeling reveals the metabolic potential of three Type II methanotrophs of the genus Methylocystis
A1 Bordel Velasco, Sergio
A1 Rodríguez Muñoz, Yadira
A1 Hakobyan, Anna
A1 Rodríguez Rodríguez, Elisa
A1 Lebrero Fernández, Raquel
A1 Muñoz Torre, Raúl
K1 Genome
K1 Genoma
AB Genome Scale Metabolic Models (GSMMs) of the recently sequenced Methylocystis hirsuta and two other methanotrophs from the genus Methylocystis have been reconstructed. These organisms are Type II methanotrophs with the ability of accumulating Polyhydroxyalkanoates under nutrient limiting conditions. For the first time, GSMMs have been reconstructed for Type II methanotrophs. These models, combined with experimental biomass and PHB yields of Methylocystis hirsuta, allowed elucidating the methane oxidation mechanism by the enzyme pMMO (particulate methane monooxygenase) in these organisms. In contrast to Type I methanotrophs, which use the “direct coupling mechanism”, Type II methanotrophs appear to use the so called “redox arm mechanism”. The utilization of the “redox arm mechanism”, which involves the coupling between methane oxidation and complex I of the respiratory chain, was confirmed by inhibition of complex I with catechol. Utilization of the “redox arm” mechanism leads to lower biomass yields on methane compared to Type I methanotrophs. However, the ability of Type II methanotrophs to redirect high metabolic carbon fluxes towards acetoacetyl-CoA under nitrogen limiting conditions makes these organisms promising platforms for metabolic engineering.
PB Elsevier
SN 1096-7176
YR 2019
FD 2019
LK http://uvadoc.uva.es/handle/10324/38025
UL http://uvadoc.uva.es/handle/10324/38025
LA eng
NO Metabolic Engineering Volume 54, 2019, Pages 191-199
NO Producción Científica
DS UVaDOC
RD 24-abr-2024