RT info:eu-repo/semantics/article
T1 Comparison study between colorimetric method and flame atomic absorption spectrophotometry in serum zinc status
A1 Escobedo Monge, Marlene Fabiola
A1 Barrado Esteban, Enrique
A1 Alonso Vicente, Carmen
A1 Marugán de Miguelsanz, José Manuel
K1 Serum zinc concentration, hypozincemia, Colorimetric method, flame atomic absorption spectrophotometry.
AB Introduction: Zinc is an essential trace element for humanlife and its deficiency affects human growth and development.Serum zinc concentration (SZnC) provides useful informationin the clinical categorization of deficiency andtoxicity states.Objectives: This paper presents a comparison study ofFlame Atomic Absorption Spectrometry (FASS) and theColorimetric method in the analysis of SZnC and hypozincemia.Methods: The serum concentrations of zinc of 93 patients(1 to 31 years old) with chronic diseases were used for analysis.Statistical analytical: for SZnC, Pearson correlation coefficient,simple linear regression analysis, Bland & Altmanmethod (B&A), and concordance correlation coefficient (CCC)were valued; and for hypozincemia, the differences werestudied and Cohen’s Kappa index was used.Results: The main results indicate the means of the SZnCby both methods presented neither significant difference(p=0.328) nor linear relation (R=0.18, p=0.077). Furthermore,the percentage of cases of hypozincemia by theColorimetric method was almost double (13%) than by theFASS (8%). There was only one coincident case in both methodsat <70 μg/dl.Discussion: The Colorimetric method in hypozincemiaranges predicted lower values with the FASS. This concordancepoor between both methods was corroborated with aconcordance correlation coefficient (CCC) lowly of 0.17.Moreover, the Cohen’s Kappa index (-0.013) shown a concordancepoor between both methods, too. In other studies, thevariability of SZnC by Colorimetric method is more than FASS.Conclusion: In summary, despite that, the mean ofserum concentrations of zinc by both methods is similar;the diagnosis of cass with hypozincemia is not. The degreeof agreement between methods is poor, with a poorstrength of concordance to diagnosis hypozincemia.Therefore, we recommended the use of FASS to evaluatedzinc status and diagnosis of hypozincemia, instead of theColorimetric method.
PB SEDCA
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/38722
UL http://uvadoc.uva.es/handle/10324/38722
LA spa
NO NUTRICIÓN CLÍNICA Y DIETÉTICA HOSPITALARIA, 38(2) (2018) 128-133
DS UVaDOC
RD 23-abr-2024