RT info:eu-repo/semantics/article T1 Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure A1 Salvo Comino, Coral A1 González Gil, Alfonso A1 Rodríguez Valentín, Javier A1 García Hernández, Celia A1 Martín Pedrosa, Fernando A1 García Cabezón, Ana Cristina A1 Rodríguez Méndez, María Luz K1 Catechol K1 Catecol K1 Gold nanoparticles K1 Nanopartículas de oro K1 Laccases K1 Lacasas K1 Tyrosinase K1 Tirosinasa K1 2307 Química Física K1 2303 Química Inorgánica AB Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors. Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP). Films with different layer structures were successfully formed. Characterization of surface roughness and porosity was carried out using AFM. Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform. LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs. The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10−4 μM, which was one order of magnitude lower than the 9.55·10−3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms. It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances. PB MDPI SN 1424-8220 YR 2020 FD 2020 LK http://uvadoc.uva.es/handle/10324/40943 UL http://uvadoc.uva.es/handle/10324/40943 LA eng NO Sensors, 2020, vol. 20, n. 7. 12 p. NO Producción Científica DS UVaDOC RD 28-mar-2024