RT info:eu-repo/semantics/article
T1 Age and sex-adjusted reference intervals in tear cytokine levels in healthy subjects
A1 Fernández Martínez, Itziar
A1 Enriquez De Salamanca Aladro, Amalia
A1 Portero, Alejandro
A1 García Vázquez, Carmen
A1 Calonge, Margarita
A1 Herreras Cantalapiedra, José María
K1 Ophthalmology
K1 Ojo Enfermedades y defectos
K1 Tear cytokines
K1 Biomarkers
K1 Age- and sex-influence
K1 Dry eye disease
K1 Citocinas lagrimales
K1 Biomarcadores
K1 Influencia de la edad y el sexo
K1 Enfermedad del ojo seco
K1 3201.09 Oftalmología
AB Alterations in tear cytokine levels have been associated with various ocular disorders as compared to those in healthy subjects. However, age and sex are not always considered in these comparisons. In this study we aimed to establish age and sex reference intervals (RIs) for tear cytokine levels in healthy people. Tear samples were taken from 75 males and 82 females, aged 18–88 years, and tear cytokine levels were determined. Age- and sex-adjusted RIs for epidermal growth factor (EGF), fractalkine, interleukin (IL)-1 receptor antagonist (RA), IL-7, IL-8, interferon inducible protein (IP)-10, monocyte chemotactic protein (MCP)-1, and vascular endothelial growth factor (VEGF) tear cytokine levels in a healthy sample were established using generalized additive for location, scale and shape (GAMLSS) models. RIs were tested in two external samples: a validation sample of 40 individuals with normal results at four Dry Eye Disease (DED) clinical diagnostic tests (OSDI, T-BUT, corneal staining and Schirmer test); and a utility sample of 13 severe DED cases. IL-1RA, IL-8, IP-10, and MCP-1 levels showed a positive association with age, while EGF was negatively correlated. IL-7 concentration increased up to 40 years and again after 70 years, observing a quasi-linear decrease between them. For VEGF, higher levels were observed in the middle-aged range. Regarding sex-influence, fractalkine tear levels were higher in men, whereas those of IL-7, IL-8, and IP-10 were higher in women. Using the estimated age- and sex-adjusted RIs, more than 92% of the validation sample was correctly classified, and 100% of the severe DED patients in the utility sample had concentrations outside the RIs in at least two of the cytokines evaluated.
PB MDPI
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/59264
UL https://uvadoc.uva.es/handle/10324/59264
LA eng
NO Applied Science, 2021, vol. 11, n. 19, 8958
NO Producción Científica
DS UVaDOC
RD 22-may-2024