RT info:eu-repo/semantics/article
T1 Apolipoprotein D alters the early transcriptional response to oxidative stress in the adult cerebellum
A1 Bajo Grañeras, Raquel
A1 Sánchez Romero, Diego
A1 Gutiérrez, Gabriel
A1 González, Constancio
A1 Carmo, Sonia do
A1 Rassart, Eric
A1 Ganfornina Álvarez, María Dolores
K1 Nervioso, sistema - Fisiología
AB The lipocalin Apolipoprotein D (ApoD), known to protect thenervous system against oxidative stress (OS) in modelorganisms, is up-regulated early in the mouse brain inresponse to the ROS generator paraquat. However, the processestriggered by this up-regulation have not been explored.We present here a study of the effect of ApoD on the earlytranscriptional changes upon OS in the mouse cerebellumusing microarray profiling. ApoD-KO and transgenic miceover-expressing ApoD in neurons are compared to wild-typecontrols. In control conditions, ApoD affects the transcriptionalprofile of neuron and oligodendrocyte-specific genes involvedin neuronal excitability, synaptic function, and myelinhomeostasis. When challenged with paraquat, the absence ofApoD modifies the response of genes mainly related to OSmanagement and myelination. Interestingly, the over-expressionof ApoD in neurons almost completely abolishes the earlytranscriptional response to OS. We independently evaluatethe expression of protein kinase Cd, a gene up-regulated byOS only in the ApoD-KO cerebellum, and find it overexpressedin cultured ApoD-KO primary astrocytes, whichpoints to a role for ApoD in astrocyte-microglia signaling. Ourresults support the hypothesis that ApoD is necessary for aproper response of the nervous system against physiologicaland pathological OS.
PB International Society for Neurochemistry
SN 0022-3042
YR 2011
FD 2011
LK http://uvadoc.uva.es/handle/10324/6099
UL http://uvadoc.uva.es/handle/10324/6099
LA eng
NO Journal of Neurochemistry, 2011, vol. 117, p. 949-960
NO Producción Científica
DS UVaDOC
RD 24-abr-2024