RT info:eu-repo/semantics/article
T1 in vivo toxicity of the ribosome-inactivating lectin ebulin f in elderly mice
A1 Garrosa, Manuel
A1 Jimenez, Pilar
A1 Córdoba-Díaz, Damián
A1 García-Recio, Verónica
A1 Gayoso, Sara
A1 Rojo, María Ángeles
A1 Gayoso, Manuel J.
A1 Girbes, Tomás
K1 Ebulin
K1 Ribosome-inactivating protein
K1 RIP
K1 RIL
K1 Lectin
AB Ebulin f is a ribosome-inactivating protein (RIP) present in green fruits of the dwarf elder (Sambucus ebulus L). Since dwarf elder fruits are used for food and as a medicine, we assessed the study of toxicological effects and safety of ebulin f in elderly mice, comparing these results with those reported in young animals and with other RIPs. Female Swiss mice aged 6 and 12 months of age were intraperitoneally injected with a single dose from 1.4 to 4.5 mg/kg ebulin f. Heart, stomach, intestines, lung, kidney, liver, spleen, pancreas, adrenal gland, uterus, ovary and brain were studied. Histology analysis was carried out by staining with hematoxylin and eosin and Masson's trichrome observed with a light microscope, or apoptosis detection by TUNEL method observed with a confocal laser microscope. Treated animals injected with the lower dose could recover their weights, but after 14 days half of them died. The higher dose caused a progressive loss of body weight leading to death. In the animals of the experimental groups it was found atrophy of Lieberkühn's crypts, pneumonia, nephronal degeneration, myocardial atrophy, centrolobular hepatic necrosis, splenic white pulp necrosis foci and increased rate of apoptosis in the intestines and liver, in which apoptoses were mainly located in the vicinity of the lobular central vein. We conclude that ebulin f affects vital organs in elderly mice.
PB Juan F.Madrid, Editor in Chief
SN 0213-3911
YR 2017
FD 2017
LK https://uvadoc.uva.es/handle/10324/64345
UL https://uvadoc.uva.es/handle/10324/64345
LA eng
NO Histology and Histopathology, 2018 vol. 33, p.979-986
NO Producción Científica
DS UVaDOC
RD 12-may-2024