RT info:eu-repo/semantics/article
T1 Chemical constituents and antimicrobial activity of a Ganoderma lucidum (Curtis.) P. Karst. Aqueous ammonia extract
A1 Sánchez Hernández, Eva
A1 Teixeira, Ana
A1 Pereira, Catarina
A1 Cruz, Adriana
A1 Martín Gil, Jesús
A1 Oliveira, Rui
A1 Martín Ramos, Pablo
K1 Antifungal activity
K1 Chitosan
K1 Gas chromatography
K1 Cromatografía de gases
K1 Mass spectrometry
K1 Espectrometría de masas
K1 Vegetable extracts
K1 Mushrooms
K1 Natural products
K1 Productos naturales
K1 Phytopathogens
K1 Microorganismos fitopatógenos
K1 Encinas
K1 Ecology
K1 Plant Science
K1 3101.05 Fungicidas
K1 3108.05 Hongos
K1 2417.13 Ecología Vegetal
K1 3102 Ingeniería Agrícola
K1 3106 Ciencia Forestal
AB Mushroom extracts have shown potential as a source of new antimicrobial agents. This study investigates the chemical profile of an aqueous ammonia extract obtained from the carpophores of Ganoderma lucidum, which grows on Quercus ilex trees, and explores its valorization as a biorational. The major chemical constituents of the extract, identified through gas chromatography–mass spectrometry, include acetamide, oleic acid, 1,2,3,4-butanetetrol, monomethyl azelate, undecane, and palmitic acid. The anti-oomycete and antifungal activity of G. lucidum extract was evaluated against Phytophthora cinnamomi, the primary threat to Quercus spp. in the dehesa biome, as well as three Botryosphaeriaceae fungi. In vitro tests revealed minimum inhibitory concentration (MIC) values of 187.5 μg·mL−1 against P. cinnamomi and 187.5–1000 μg·mL−1 against the fungi. Furthermore, conjugation of the G. lucidum extract with chitosan oligomers (COS) synergistically enhanced its antimicrobial activity, resulting in MIC values of 78.12 and 375–500 μg·mL−1 against P. cinnamomi and the fungi, respectively. These MIC values are among the highest reported to date for natural products against these phytopathogens. Subsequent ex situ testing of the COS-G. lucidum conjugate complex on artificially inoculated Q. ilex excised stems resulted in high protection against P. cinnamomi at a dose of 782 µg·mL−1. These findings support the potential utilization of this resource from the dehesa ecosystem to protect the holm oak, aligning with sustainable and circular economy approaches.
PB MDPI
SN 2223-7747
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/65892
UL https://uvadoc.uva.es/handle/10324/65892
LA eng
NO Plants, 2023, Vol. 12, Nº. 12, 2271
NO Producción Científica
DS UVaDOC
RD 17-may-2024