RT info:eu-repo/semantics/article
T1 Chemical compounds causing severe acute toxicity in heavy liquids used for intraocular surgery
A1 Coco Martín, Rosa María
A1 Srivastava, Girish Kumar
A1 Andrés Iglesias, Cristina
A1 Fernández Bueno, Iván
A1 Medina García, Jesús
A1 García Serna, Juan
A1 Dueñas Laita, Antonio
A1 Rull Pérez, Fernando
A1 Pastor Jimeno, José Carlos
K1 Cytotoxicity
K1 Retina
K1 Gas-chromatography
K1 Mass spectrometry
K1 FTIR
K1 Perfluorooctanoic acid
K1 ARPE-19
K1 Perfluorooctane
K1 Perfluorohexyl-octane
K1 Tributyltin bromide
AB Perfluorocarbon liquids (PFCLs) have been considered safe for intraocular manipulation of the retina, but since 2013 many cases of acute eye toxicity cousing blindness have been reported in various countries when using various commercial PFCLs. All these PFCLs were CE marked (Conformité Européenne), which meant they had been subjected to evaluation complying with the International Organization for Standardization (ISO) guidelines. These dramatic events raised questions about the safety of PFCLs and the validity of some cytotoxicity tests performed under ISO guidelines. Samples from toxic batches were analyzed by gas chromatography-mass spectrometry combined with Raman and infrared spectrometry. Perfluorooctanoic acid, dodecafluoro-1-heptanol, ethylbenzene and tributyltin bromide were identified and evaluated by a direct contact cytotoxicity test using ARPE-19 cell line, patented by our group (EP 3467118 A1). Perfluorooctanoic acid at a concentration of >0.06 mM and tributyltin bromide at a concentration of ≥0.016 mM were shown to be toxic, whereas the concentration found in the toxic samples reached 0.48 mM, and 0.111 mM, respectively. These finding emphasized the idea that determination of partially fluorinated compounds are not enough to guarantee the safety of these medical devices.
PB ELSEVIER SCIENCE
SN 0273-2300
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/67013
UL https://uvadoc.uva.es/handle/10324/67013
LA eng
NO Chemical compounds causing severe acute toxicity in heavy liquids used for intraocular surgery, feb., 2020, vol.110, nº Art: 104527 (8 pag)
NO Producción Científica
DS UVaDOC
RD 21-may-2024