RT info:eu-repo/semantics/article
T1 Spermine attenuates carotid body glomus cell oxygen sensing by inhibiting
A1 Cayzac, A.
A1 Rocher Martín, María Asunción
A1 Obeso Cáceres, Ana María de la Luz
A1 González, Constancio
A1 Ricardi, D.
A1 Kemp, P. K.
K1 Respiración celular
AB An increase in intracellular Ca2+ is crucial to O2 sensing by the carotid body. Polyamines have beenreported to modulate both the extracellular Ca2+-sensing receptor (CaR) and voltage-gated Ca2+ channelsin a number of cell types. Using RT-PCR and immunohistochemistry, the predominant voltage-gated Ca2+channels expressed in the adult rat carotid body were L (CaV1.2) and N (CaV2.2)-type. CaR mRNA couldnot be amplified from carotid bodies, but the protein was expressed in the nerve endings. Spermineinhibited the hypoxia-evoked catecholamine release from isolated carotid bodies and attenuated thedepolarization- and hypoxia-evoked Ca2+ influx into isolated glomus cells. In agreement with data fromcarotid body, recombinant CaV1.2 was also inhibited by spermine. In contrast, the positive allostericmodulator of CaR, R-568, was without effect on hypoxia-induced catecholamine release from carotidbodies and depolarization-evoked Ca2+ influx into glomus cells. These data show that spermine exerts anegative influence on carotid body O2 sensing by inhibiting L-type Ca2+ channels.
PB Elsevier
SN 1569-9048
YR 2011
FD 2011
LK http://uvadoc.uva.es/handle/10324/7091
UL http://uvadoc.uva.es/handle/10324/7091
LA eng
NO Respiratory Physiology & Neurobiology 175 (2011) 80–89
NO Innovación Educativa
DS UVaDOC
RD 19-abr-2024