RT info:eu-repo/semantics/article T1 Valorization of CH4 emissions into high-added-value products: Assessing the production of ectoine coupled with CH4 abatement A1 Cantera Ruiz de Pellón, Sara A1 Lebrero Fernández, Raquel A1 Sadornil Delgado, Lidia Jimena A1 García Encina, Pedro Antonio A1 Muñoz Torre, Raúl K1 Gases de efecto invernadero K1 Metano AB This study assessed an innovative strategy for the valorization of dilute methane emissions based on the bio-conversion of CH4 (the second most important greenhouse gas (GHG)) into ectoine by the methanotrophic ectoine-producing strain Methylomicrobium alcaliphilum 20 Z. The influence of CH4 (2–20%), Cu2+ (0.05–50 μM) and NaCl (0–9%) concentration as well as temperature (25–35 °C) on ectoine synthesis and specific CH4 biodegradation rate was evaluated for the first time. Concentrations of 20% CH4 (at 3% NaCl, 0.05 μM Cu2+, 25 °C) and 6% NaCl (at 4% CH4, 0.05 μM Cu2+, 25 °C) supported the maximum intra-cellular ectoine production yield (31.0 ±1.7 and 66.9 ±4.2 mg g biomass−1, respectively). On the other hand, extra-cellular ectoine concentrations of up to 4.7 ± 0.1 mg L−1 were detected at high Cu2+concentrations (50 μM), despite this methanotroph has not been previously classified as an ectoine-excreting strain. This research demonstrated the feasibility of the bio-conversion of dilute emissions of methane into high-added value products in an attempt to develop a sustainable GHG bioeconomy. PB Elsevier YR 2016 FD 2016 LK http://uvadoc.uva.es/handle/10324/23455 UL http://uvadoc.uva.es/handle/10324/23455 LA eng NO Journal of Environmental Management, 2016, Volume 182, p. 160–165 NO Producción Científica DS UVaDOC RD 30-abr-2024