RT info:eu-repo/semantics/article
T1 High Levels of Proinflammatory Cytokines, but not markers of tissue injury in unaffected intestinal areas from patients with IBD
A1 León, Alberto José
A1 Gómez, Emma
A1 Garrote Adrados, José Antonio
A1 Bernardo Ordiz, David
A1 Barrera, Asterio
A1 Marcos, José Luis
A1 Fernández Salazar, Luis Ignacio
A1 Velayos Jiménez, Benito
A1 Blanco Quirós, Alfredo
A1 Arranz Sanz, Eduardo
K1 Intestinos - Enfermedades
K1 Enfermedad de Chrom
AB Intestinal alterations in IBD are triggered and maintained by an overexpression of proinflammatory cytokines. Additionally,increased immune activation has been found in the adjacent intestinal areas without displaying any apparent histologicalalterations, however, the regulatory environment is not well established. Biopsy specimens from patients with ulcerative colitis(UC) and Crohn’s disease (CD), from both affected and unaffected areas, and also from a group of colonic biopsies from healthycontrols, were included in our study. Cytokines and markers of mucosal damage were analyzed by real-time PCR, and some of theresults confirmed by western-blot and ELISA. Levels of IFNγ, TNFα, IL-6, IL-15, IL-18, and IL-23 were increased (above healthycontrols) in both affected and unaffected areas from IBD. IL-1β, IL-6, IL-12, and IL-27 were higher in affected areas compared tounaffected ones in UC but not CD. In general, a correlation was observed between mRNA levels of these cytokines and both iNOSand Granzyme B. SOCS-2 and SOCS-3 were also increased in the affected areas. In conclusion, the unaffected areas from IBD showincreased levels of a restricted set of cytokines that may exert immune activating roles in these areas without being able to triggertissue damage.
PB Hindawi Publishing Corporation
SN 0962-9351
YR 2009
FD 2009
LK http://uvadoc.uva.es/handle/10324/4149
UL http://uvadoc.uva.es/handle/10324/4149
LA eng
NO Mediators of inflammation, June 2009 p. 1-10
NO Producción Científica
DS UVaDOC
RD 28-abr-2024