RT info:eu-repo/semantics/article
T1 Elastin-plasma hybrid hydrogels for skin tissue engineering
A1 Stojic, Marija
A1 Ródenas Rochina, Joaquín
A1 López Donaire, María Luisa
A1 González de Torre, Israel
A1 González Pérez, Miguel
A1 Rodríguez Cabello, José Carlos
A1 Vojtová, Lucy
A1 Jorcano Noval, José Luis
A1 Velasco Bayón, Diego
K1 Elastin
K1 Elastin like recombinamers
K1 Fibrin hydrogels
K1 Elastin-plasma hybrid hydrogels
K1 Skin tissue engineering
K1 Bioengineered skin
K1 22 Física
K1 23 Química
K1 24 Ciencias de la Vida
AB Dermo-epidermal equivalents based on plasma-derived fibrin hydrogels have been ex tensively studied for skin engineering. However, they showed rapid degradation and contractionover time and low mechanical properties which limit their reproducibility and lifespan. In orderto achieve better mechanical properties, elasticity and biological properties, we incorporated aelastin-like recombinamer (ELR) network, based on two types of ELR, one modified with azide(SKS-N3) and other with cyclooctyne (SKS-Cyclo) chemical groups at molar ratio 1:1 at three differentSKS (serine-lysine-serine sequence) concentrations (1, 3, and 5 wt.%), into plasma-derived fibrinhydrogels. Our results showed a decrease in gelation time and contraction, both in the absence andpresence of the encapsulated human primary fibroblasts (hFBs), higher mechanical properties andincrease in elasticity when SKSs content is equal or higher than 3%. However, hFBs proliferationshowed an improvement when the lowest SKS content (1 wt.%) was used but started decreasingwhen increasing SKS concentration at day 14 with respect to the plasma control. Proliferation ofhuman primary keratinocytes (hKCs) seeded on top of the hybrid-plasma hydrogels containing 1and 3% of SKS showed no differences to plasma control and an increase in hKCs proliferation wasobserved for hybrid-plasma hydrogels containing 5 wt.% of SKS. These promising results showedthe need to achieve a balance between the reduced contraction, the better mechanical properties andbiological properties and indicate the potential of using this type of hydrogel as a testing platformfor pharmaceutical products and cosmetics, and future work will elucidate their potential.
PB MDPI
SN 2073-4360
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/53937
UL https://uvadoc.uva.es/handle/10324/53937
LA eng
NO Polymers, 2021, vol. 13, n. 13, p. 2114
NO Producción Científica
DS UVaDOC
RD 10-nov-2024