RT info:eu-repo/semantics/article
T1 Functionalization of 3D-printed titanium scaffolds with elastin-like recombinamers to improve cell colonization and osteoinduction
A1 Guillem Marti, Jordi
A1 Vidal, Elia
A1 Girotti, Alessandra
A1 Heras Parets, Aina
A1 Torres, Diego
A1 Arias Vallejo, Francisco Javier
A1 Ginebra, M. P.
A1 Rodríguez Cabello, José Carlos
A1 Manero, José Maria
K1 3D Printing
K1 Three-dimensional printing
K1 Impresión tridimensional
K1 Imágenes tridimensionales en medicina
K1 Diseño asistido por ordenador
K1 Polymers in medicine
K1 Polímeros recombinantes
K1 Polímeros - Aplicaciones médicas
K1 Biomaterials
K1 Biomateriales
K1 Osseointegration
K1 Titanium
K1 Pharmaceutical technology
K1 Pharmacology
K1 Functionalization
K1 2206.10 Polímeros
K1 2415 Biología Molecular
K1 3209 Farmacología
K1 3312 Tecnología de Materiales
AB The 3D printing of titanium (Ti) offers countless possibilities for the development of personalized implants with suitable mechanical properties for different medical applications. However, the poor bioactivity of Ti is still a challenge that needs to be addressed to promote scaffold osseointegration. The aim of the present study was to functionalize Ti scaffolds with genetically modified elastin-like recombinamers (ELRs), synthetic polymeric proteins containing the elastin epitopes responsible for their mechanical properties and for promoting mesenchymal stem cell (MSC) recruitment, proliferation, and differentiation to ultimately increase scaffold osseointegration. To this end, ELRs containing specific cell-adhesive (RGD) and/or osteoinductive (SNA15) moieties were covalently attached to Ti scaffolds. Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SNA15-ELR. The combination of both RGD and SNA15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. These results suggest that biofunctionalization with SNA15-ELRs could modulate the cellular response to improve the osseointegration of Ti implants. Further investigation on the amount and distribution of RGD and SNA15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study.
PB MDPI
SN 1999-4923
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/63729
UL https://uvadoc.uva.es/handle/10324/63729
LA eng
NO Pharmaceutics, 2023, Vol. 15, Nº. 3, 872
NO Producción Científica
DS UVaDOC
RD 20-may-2024