RT info:eu-repo/semantics/article
T1 Histological and immunohistochemical characterization of the porcine ocular surface
A1 Crespo Moral, Mario
A1 García Posadas, Laura
A1 López García, Antonio
A1 Diebold Luque, María Yolanda
K1 Oftalmología
K1 Porcine eyeballs
K1 Ocular surface
K1 Globos oculares porcinos
K1 Superficie ocular
K1 3201.09 Oftalmología
AB The ocular surface of the white domestic pig (Sus scrofa domestica) is used as a helpful model of the human ocular surface; however, a complete histological description has yet to be published. In this work, we studied porcine eyeballs with intact eyelids to describe and characterize the different structures that form the ocular surface, including the cornea and conjunctiva that covers the bulbar sclera, tarsi, and the nictitating membrane. We determined the distribution of goblet cells of different types over the conjunctiva and analyzed the conjunctival-associated lymphoid tissue (CALT). Porcine eyeballs were obtained from a local slaughterhouse, fixed, processed, and embedded in paraffin blocks. Tissue sections (4 μm) were stained with hematoxylin/eosin, Alcian blue/Periodic Acid Schiff, and Giemsa. Slides were also stained with lectins from Arachis hypogaea (PNA) and Helix pomatia (HPA) agglutinins and immunostained with rabbit anti-CD3. We found that the porcine cornea was composed of 6–8 epithelial cell layers, stroma, Descemet’s membrane, and an endothelial monolayer. The total corneal thickness was 1131.0±87.5 μm (mean±standard error of the mean) in the center and increased to 1496.9±138.2 μm at the limbus. The goblet cell density was 71.25±12.29 cells/mm, ranging from the highest density (113.04±37.21 cells/mm) in the lower palpebral conjunctiva to the lowest density (12.69±4.29 cells/mm) in the bulbar conjunctiva. The CALT was distributed in the form of intraepithelial lymphocytes and subepithelial diffuse lymphoid tissue. Lenticular-shaped lymphoid follicles, about 8 per histological section, were also present within the conjunctival areas. In conclusion, we demonstrated that the analyzed porcine ocular structures are similar to those of humans, confirming the potential usefulness of pig eyes to study ocular surface physiology and pathophysiology
PB PLoS ONE
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/64991
UL https://uvadoc.uva.es/handle/10324/64991
LA eng
NO PLoS ONE, 2020, vol. 15, n.1, e0227732
NO Producción Científica
DS UVaDOC
RD 20-may-2024