This BITE_437_133082_Readme.txt was generated on 2025-27-08 by Víctor Rodríguez Gallego INDEX OF THE BITE_437_133082 DATASET 1. GENERAL INFORMATION 1.1. Title of dataset 1.2. Author information 1.2.1. PI and co-PI 1.2.2. Labs 1.2.3. People involved in the data collection 1.3. Dataset description 1.4. Funding sources 1.5. Citing information 2. ACCESS INFORMATION 2.1. Licenses or restrictions 2.2. Publications 3. METHODOLOGICAL INFORMATION 3.1. Experimental setup and data collection procedure 4. DATA 4.1. Inventory of data files 4.2. Last update 1. GENERAL INFORMATION 1.1. Title of dataset: BITE_437_133082 1.2. Author Information 1.2.1. Authors data: Name: Víctor Rofríguez Gallego Institution: University of Valladolid (Spain) Address: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Paseo del Prado de la Magdalena 3-5, 47011 Valladolid, Spain Institution: Institute of Sustainable Processes Address: University of Valladolid, Paseo del Prado de la Magdalena 3-5, 47011 Valladolid, Spain Name: Paula Bucci Institution: University of Valladolid (Spain) Address: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Paseo del Prado de la Magdalena 3-5, 47011 Valladolid, Spain Institution: Institute of Sustainable Processes Address: University of Valladolid, Paseo del Prado de la Magdalena 3-5, 47011 Valladolid, Spain Name: Raquel Lebrero Institution: University of Valladolid (Spain) Address: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Paseo del Prado de la Magdalena 3-5, 47011 Valladolid, Spain Institution: Institute of Sustainable Processes Address: University of Valladolid, Paseo del Prado de la Magdalena 3-5, 47011 Valladolid, Spain Name: Raúl Muñoz Institution: University of Valladolid (Spain) Address: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Paseo del Prado de la Magdalena 3-5, 47011 Valladolid, Spain Institution: Institute of Sustainable Processes Address: University of Valladolid, Paseo del Prado de la Magdalena 3-5, 47011 Valladolid, Spain Email: raul.munoz.torre@uva.es 1.2.2. Lab: Name of the lab: ISP (Institute of Sustainable Processes) Institution: University of Valladolid (Spain) Address: https://isp.uva.es Email: isp@uva.es 1.2.3. People involved in the data collection The collection of the experimental data were done by Víctor Rodríguez Gallego and Paula Bucci. 1.3. Corpus description The dataset compiled in this spreadsheet contains the experimental data used for the development and analysis presented in the associated research paper, regarding the evaluation of bioreactors performance and activity tests. The corpus includes measurements and observations obtained from two experimental setups aimed at investigating syngas biomethanation and the involved metabolic pathways: the continuous bioreactor experiments and batch activity tests in serum bottles. - Bioreactor Experimental Data [Inoculum, BCB1 and BCB2] This section documents the operation of two lab-scale bioreactors for syngas biomethanation under different operational conditions. The dataset includes: - Inoculum characterization - Sampling dates and experimental timepoints - Reactor operating conditions - Inlet and outlet gas compositions (H₂, CO, CO₂, CH₄, O₂, N₂) - TOC/TN evolution - TS/VS evolution - VFAs concentration measurements - pH evolution - Serum Bottle Activity Tests [M. activity tests, T. activity tests and Activity tests summary] This section contains the results of batch tests conducted in sealed serum bottles to evaluate the metabolic activity of microbial cultures under varying substrates addition. The dataset includes: - Assay type (regarding substrates employed) - Initial and final pH measurements - Headspace pressure measurements - Headspace gas measurements (CO, H₂, CO₂, CH₄, O₂, N₂) - Incubation temperture conditions - VFAs concentration measurements - Calculated product yields The data provided insight into microbial activity under the addition of different substrates and were used to evaluate and identify the metabolic pathways involved in the developed syngas biomethanation process. 1.4. Funding sources - Spanish Ministry of Science and Innovation (PLEC2022-009349 and PID2021-124347OB-I00). - Regional Government of Castilla y León (JCyL) and the FEDER program (UIC379 and UIC393). 1.5. Citing information Publications using this dataset (or any part of it) should cite this dataset as follows: Rodríguez-Gallego, V., Bucci, P., Lebrero, R., & Muñoz, R. (2025). Enrichment of mesophilic and thermophilic mixed microbial cultures for syngas biomethanation in bubble column bioreactors under continuous operation. Bioresource Technology, 437, 133082. 2. ACCESS INFORMATION 2.1. Licenses or restrictions There are no licenses/restrictions placed on the data. 2.2. Publications The associated publication of this dataset can be found at https://doi.org/10.1016/j.biortech.2025.133082 3. METHODOLOGICAL INFORMATION 3.1. Experimental setup and data collection procedure The methodology for data collection is widely explained in the section 2. Materials and methods of the corresponding paper published in Bioresource Technology: Enrichment mesophilic and thermophilic mixed microbial cultures for syngas biomethanation in bubble column bioreactors under continuous operation (https://doi.org/10.1016/j.biortech.2025.133082). 4. DATA 4.1. Inventory of data files The inventory of the files in the repository appears in the following Excel file: BITE_437_133082.xlsx. The information in the inventory is divided into six parts: 1) Inoculum: Inoculum characterization data. 2) BCB1: Mesophilic syngas biomethanation biorreactor experimental data collected. 3) BCB2: Thermophilic syngas biomethanation biorreactor experimental data collected. 4) M. activity tests: Mesophilic activity tests experimental data collected and calculations. 5) T. activity tests: Thermophilic activity tests experimental data collected and calculations. 6) Activity tests summary: Summary of data and calculations 4.2. Last update: 2025