Dpto. Anatomia y Radiología - Capítulos de monografías https://uvadoc.uva.es/handle/10324/1206 2026-04-12T19:09:05Z 2026-04-12T19:09:05Z Alonso Revuelta, María Isabel Martínez Páramo, Sonia Lamus Molina, José Francisco Gato Casado, Ángel Luis https://uvadoc.uva.es/handle/10324/76356 2025-07-15T19:07:52Z 2025-01-01T00:00:00Z

Adult brain neural precursors carry out their biological activity in specific areas in which they are able to self-renew and differentiate into neurons. This is due to a complex microenvironment of cellular interrelations in which soluble factors from the neighboring cells, vascular structures, and the content of the brain ventricle cavity (cerebrospinal fluid) play a key role. This cellular functional entity, known as the “neurogenic niche,” is able to generate new mature neurons, which are functionally integrated into the neuronal circuits of the adult mammal brain. The complexity of neurogenic niche signaling, which include biologically active molecules such as growth factors and morphogens, requires an experimental approach in order to create specific modifications of the biological activity of some of these molecules by means of a model of the active neurogenic niche, allowing an evaluation of neural precursor behavior. Here we describe the adaptation of an in vitro culture technique of adult brain slices with selected coronal sections, involving the two main brain neurogenic niches, the sub-ventricular zone (SVZ), and the hippocampus dentate gyrus, together with their associated sub-ependymal zone (SEZ). We explain certain examples of the experimental approach to modify neurogenic niche soluble signaling, implanting latex microbeads as a carrier for soluble signals. Additionally, we introduce an immune-cytochemical approach involving bromodeoxyuridine detection as a neural precursor cellular lineage tracer in combination with different molecular expressions, as a means of testing progressive states of neural precursor differentiation and neuronal maturation. This system represents a suitable strategy for evaluating the biological role of soluble components of the adult brain neurogenic niche.

2025-01-01T00:00:00Z Sainz Esteban, Aurora Esteban Casado, Rosario Trueba Arguiñarena, Francisco Javier https://uvadoc.uva.es/handle/10324/74722 2025-01-31T20:00:41Z 2021-01-01T00:00:00Z

La Radiología ha evolucionado significativamente en el ámbito médico, pasando de ser una disciplina complementaria en el siglo XX a convertirse en un pilar fundamental para la toma de decisiones clínicas en el siglo XXI. Esto se refleja en el creciente protagonismo de la especialidad en las convocatorias recientes del examen MIR. Sin embargo, a pesar de su importancia, muchos planes de estudio universitarios continúan relegándola a un papel secundario. Siguiendo la línea de publicaciones previas como Radiología Esencial, que sirvieron como referencia en la formación de residentes en Radiodiagnóstico, la sección de Formación en Radiología de la SERAM presenta este material adaptado a estudiantes de Medicina. Su objetivo es cubrir una necesidad educativa aún pendiente en la preparación del médico general. Este texto abarca los conocimientos y habilidades fundamentales que los estudiantes deben adquirir durante su formación, incluyendo aspectos técnicos, principios de radioprotección y el uso de diversas técnicas de imagen radiológica y medicina nuclear. Además, los capítulos incluyen recursos didácticos como resúmenes destacados, casos clínicos, preguntas de autoevaluación y numerosas ilustraciones que facilitan el aprendizaje.

2021-01-01T00:00:00Z Caprile, Teresa Lamus Molina, José Francisco Alonso Revuelta, María Isabel Montecinos, Hernán Gato Casado, Ángel Luis https://uvadoc.uva.es/handle/10324/74719 2025-03-11T07:59:04Z 2019-01-01T00:00:00Z

The embryonic cerebrospinal fluid (eCSF) influences neuroepithelial cell behavior, affecting proliferation, differentiation, and survival. One major question to resolve in the field is to precisely describe the eCSF molecules responsible and to understand how these molecules interact in order to exert their functions. Here we describe an in vitro protocol to analyze the influence of eCSF components on neuroepithelium development.

2019-01-01T00:00:00Z