• español
  • English
  • français
  • Deutsch
  • português (Brasil)
  • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Parcourir

    Tout UVaDOCCommunautésPar date de publicationAuteursSujetsTitres

    Mon compte

    Ouvrir une session

    Statistiques

    Statistiques d'usage de visualisation

    Compartir

    Voir le document 
    •   Accueil de UVaDOC
    • PUBLICATIONS SCIENTIFIQUES
    • Departamentos
    • Dpto. Biología Celular, Genética, Histología y Farmacología
    • DEP05 - Artículos de revista
    • Voir le document
    •   Accueil de UVaDOC
    • PUBLICATIONS SCIENTIFIQUES
    • Departamentos
    • Dpto. Biología Celular, Genética, Histología y Farmacología
    • DEP05 - Artículos de revista
    • Voir le document
    • español
    • English
    • français
    • Deutsch
    • português (Brasil)
    • italiano

    Exportar

    RISMendeleyRefworksZotero
    • edm
    • marc
    • xoai
    • qdc
    • ore
    • ese
    • dim
    • uketd_dc
    • oai_dc
    • etdms
    • rdf
    • mods
    • mets
    • didl
    • premis

    Citas

    Por favor, use este identificador para citar o enlazar este ítem:http://uvadoc.uva.es/handle/10324/10360

    Título
    Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9
    Autor
    Fuente García, Miguel Ángel de laAutoridad UVA Orcid
    Tovar, Victoria
    Villamor, Neus
    Zapater, Nuria
    Pizcueta, Pilar
    Campo, Elias
    Jaime Bosch, Jaime
    Engel, Pablo
    Año del Documento
    2001
    Editorial
    American Society of Hematology
    Descripción
    Producción Científica
    Documento Fuente
    Blood, 2001, 97(11):3513-3520
    Résumé
    .Ly-9 is a mouse cell-surface glycoprotein that is selectively expressed on thymocytes and on mature T and B lymphocytes. Ly-9 belongs to the CD2 subset of the immunoglobulin superfamily, an emerging family of cell signaling receptors. Recently, a partial human Ly-9 complementary DNA (cDNA) sequence has been described. Full-length cDNA clones were isolated that included the initiation codon, the sequence encoding the full signal peptide, and 14 amino acids more in the cytoplasmic domain than in the previously reported clone. The predicted extracellular domain of human Ly-9 contains 4 immunoglobulinlike domains, similar to those in mouse Ly-9. Northern blot analysis revealed that the human Ly-9 messenger RNA (2.6 kb) is expressed predominantly in lymph node, spleen, thymus, and peripheral blood leukocytes. Four monoclonal antibodies (mAbs) were raised against human Ly-9 by immunizing mice with the pre-B-cell line 300.19 stably transfected with human Ly-9 full-length cDNA. These mAbs strongly stained the surfaces of cells transfected with human Ly-9 cDNA but not of untransfected cells. Human Ly-9 expression was restricted to T and B lymphocytes and thymocytes, with the highest levels of expression on CD4(+)CD8(-) and CD4(-)CD8(+) thymocytes. Monocytes, granulocytes, platelets, and red blood cells were uniformly negative for Ly-9. These mAbs immunoprecipitated major polypeptides of 120 kd from the transfected cells and 120 kd and 100 kd from B-cell line Daudi, probably because of the cell-surface-expressed isoforms. These data demonstrate that human Ly-9 is a new marker for the study of normal and malignant leukocytes
    Materias (normalizadas)
    Hematología
    ISSN
    0006-4971
    Revisión por pares
    SI
    DOI
    10.1182/blood.V97.11.3513
    Idioma
    eng
    URI
    http://uvadoc.uva.es/handle/10324/10360
    Derechos
    openAccess
    Aparece en las colecciones
    • DEP05 - Artículos de revista [198]
    Afficher la notice complète
    Fichier(s) constituant ce document
    Nombre:
    18. M.A. de la Fuente PD-231.pdf
    Tamaño:
    369.3Ko
    Formato:
    Adobe PDF
    Descripción:
    PD-231
    Thumbnail
    Voir/Ouvrir
    Attribution-NonCommercial-NoDerivatives 4.0 InternationalExcepté là où spécifié autrement, la license de ce document est décrite en tant que Attribution-NonCommercial-NoDerivatives 4.0 International

    Universidad de Valladolid

    Powered by MIT's. DSpace software, Version 5.10