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dc.contributor.authorGallart, T.
dc.contributor.authorFuente García, Miguel Ángel de la es
dc.contributor.authorBarceló, Josep
dc.contributor.authorAlberola-Ila, J.
dc.contributor.authorLozano, F.
dc.date.accessioned2015-04-10T11:53:20Z
dc.date.available2015-04-10T11:53:20Z
dc.date.issued1997
dc.identifier.citationImmunology, 1997, 90(1):57-65es
dc.identifier.issn0019-2805es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/10384
dc.descriptionProducción Científicaes
dc.description.abstractActivation of T lymphocytes by pokeweed mitogen (PWM) is strictly monocyte (Mo)-dependent and results in T-cell mitogenesis and interleukin-2 (IL-2) secretion, coupled with an inability to utilize IL-2 due to an impaired expression of functional IL-2 receptor (IL-2R). Such IL-2R impairment could arise in PWM-activated T cells themselves or, alternatively, be the result of Mo-derived influences, as it is known that PWM binds Mo strongly and does not or poorly binds lymphocytes, and Mo becomes rapidly destroyed in PWM-stimulated cultures of blood mononuclear cells or T cells plus Mo. The present study investigated these possibilities. The results show for the first time that desialylation of T lymphocytes strongly increases their PWM-binding capacity and, in addition, overcomes the Mo requirement for PWM to induce T-cell mitogenesis and IL-2 secretion. Such secreted IL-2 levels were even higher that those found in cultures of Mo-dependent PWM-activated T lymphocytes but similarly to the latter, PWM-activated desialylated purified T lymphocytes exhibited negligible high-affinity IL-2 binding capacity and an inability to utilize the IL-2 they produced. These effects were not due to desialylation itself, as indicated by data obtained with peanut agglutinin, a lectin that becomes strongly reactive with desialylated T lymphocytes. The data clearly indicate the existence of PWM-related events capable of impairing the expression of functional IL-2R without affecting IL-2 secretion, and indicate that such events are due to mechanisms arising at the level of PWM-activated T cells themselves.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherWileyes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCélulas hematopoyéticases
dc.subjectHematología
dc.titleDesialylation of T lymphocytes overcomes the monocyte dependency of pokeweed mitogen-induced T-cell activation.es
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1046/j.1365-2567.1997.00129es
dc.identifier.publicationfirstpage57es
dc.identifier.publicationissue1es
dc.identifier.publicationlastpage65es
dc.identifier.publicationtitleImmunologyes
dc.identifier.publicationvolume90es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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