Comparative study of two methods for RNA extraction prior to detection of resistance to human immunodeficiency virus type 1 with the line probe assay

Eiros Bouza, José María; Labayru, Cristina; Hernández, Beatriz; Ortiz de Lejarazu Leonardo, Raúl; Rodríguez Torres, Antonio

doi:10.1128/JCM.40.6.2257-2259.2002

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Título

Comparative study of two methods for RNA extraction prior to detection of resistance to human immunodeficiency virus type 1 with the line probe assay

Autor

Eiros Bouza, José María

Labayru, Cristina

Hernández, Beatriz

Ortiz de Lejarazu Leonardo, Raúl

Rodríguez Torres, Antonio

Año del Documento

2002

Editorial

American Society for Microbiology

Descripción

Producción Científica

Documento Fuente

Journal of Clinical Microbiology, 2002 June; 40(6): 2257–2259

Résumé

We evaluated two methods from Roche and Promega for RNA extraction prior to the genotypic detection of human immunodeficiency virus type 1 resistance by line probe assay (LiPA). Fifty plasma RNA extracts were processed in parallel by LiPA. Results obtained by the Roche method were superior in the proportion of amplified samples, the percentage of mutated samples, and band intensity.

Materias (normalizadas)

Inmunodeficiencia

ISSN

0095-1137

Revisión por pares

DOI

10.1128/JCM.40.6.2257-2259.2002