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dc.contributor.authorMedina Plaza, Cristina
dc.contributor.authorSaja Sáez, José Antonio de
dc.contributor.authorRodríguez Méndez, María Luz 
dc.date.accessioned2016-11-24T16:14:18Z
dc.date.available2016-11-24T16:14:18Z
dc.date.issued2014
dc.identifier.citationBiosensors and Bioelectronics, Julio 2014, vol. 57, p. 276-283es
dc.identifier.issn09565663es
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/21104
dc.descriptionProducción Científicaes
dc.description.abstractIn this work, a multisensor system formed by nanostructured voltammetric biosensors based on phenol oxidases (tyrosinase and laccase) has been developed. The enzymes have been incorporated into a biomimetic environment provided by a Langmuir-Blodgett (LB) film of arachidic acid (AA). Lutetium bisphthalocyanine (LuPc2) has also been introduced in the films to act as electron mediator. The incorporation of the enzymes to the floating layers to form Tyr/AA/LuPc2 and Lac/AA/LuPc2 films has been confirmed by the expansion in the surface pressure isotherms and by the AFM images. The voltammetric response towards six phenolic compounds demonstrates the enhanced performance of the biosensors that resulted from a preserved activity of the tyrosinase and laccase combined with the electron transfer activity of LuPc2. Biosensors show improved detection limits in the range of 10-7-10-8molL-1. An array formed by three sensors AA/LuPc2, Tyr/AA/LuPc2 and Lac/AA/LuPc2 has been employed to discriminate phenolic antioxidants of interest in the food industry. The Principal Component Analysis scores plot has demonstrated that the multisensor system is able to discriminate phenols according to the number of phenolic groups attached to the structure. The system has also been able to discriminate grapes of different varieties according to their phenolic content. This good performance is due to the combination of four factors: the high functionality of the enzyme obtained using a biomimetic immobilization, the signal enhancement caused by the LuPc2 mediator, the improvement in the selectivity induced by the enzymes and the complementary activity of the enzymatic sensors demonstrated in the loading plots.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.subjectBisphthalocyaninees
dc.subjectElectronic tonguees
dc.subjectLaccasees
dc.subjectPhenoles
dc.subjectTyrosinasees
dc.subjectVoltammetric sensores
dc.titleBioelectronic tongue based on lipidic nanostructured layers containing phenol oxidases and lutetium bisphthalocyanine for the analysis of grapeses
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1016/j.bios.2014.02.023es
dc.identifier.publicationfirstpage276es
dc.identifier.publicationlastpage283es
dc.identifier.publicationtitleBioelectronic tongue based on lipidic nanostructured layers containing phenol oxidases and lutetium bisphthalocyanine for the analysis of grapeses
dc.identifier.publicationvolume57es
dc.peerreviewedSIes
dc.description.projectMinisterio de Economía, Industria y Competitividad – FEDER (Grant CICYT AGL2012-33535)es
dc.description.projectJunta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. VA-032U13)


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