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dc.contributor.authorIbares Frías, Lucía
dc.contributor.authorGallego Muñoz, Patricia 
dc.contributor.authorCantalapiedra Rodríguez, Roberto
dc.contributor.authorCruz Valsero, Maria Cruz
dc.contributor.authorMar Sardaña, Santiago
dc.contributor.authorMerayo Lloves, Jesús
dc.contributor.authorMartínez García, María del Carmen 
dc.date.accessioned2017-01-12T09:06:22Z
dc.date.available2017-01-12T09:06:22Z
dc.date.issued2015
dc.identifier.citationGraefe's Archive for Clinical and Experimental Ophthalmology, (2015); 253:1071–1083es
dc.identifier.issn0721-832Xes
dc.identifier.urihttp://uvadoc.uva.es/handle/10324/22085
dc.descriptionProducción Científicaes
dc.description.abstractPurpose To evaluate corneal wound healing in the hen animal model after additive surgery with an intracorneal ring segment (ICRS). Methods We implanted one ICRS in each eye of 76 hens. In control group 1 (n=22 hens), the stromal channel was prepared but no ICRS was inserted. In control group 2 (n=2 hens), no surgery was performed. Animals were randomly separated into groups and euthanized after clinical follow-up of 4 and 12 hours, 1, 2, 3, and 7 days, and 1, 2, 3, 4, and 6 months. Corneas were stained with hematoxylin-eosin. Apoptosis was measured by terminal uridine nick end-labeling assays. Cell proliferation and myofibroblast-like differentiation were assayed by BrdU and α-smooth muscle actin immunofluorescence microscopy. Stromal matrix changes were documented by electron microscopy. Results Epithelial and stromal cell apoptosis around the ICRS-implanted and control group 1 eyes peaked at 12 hours, but continued for 72 hours. In ICRSimplanted eyes, epithelial and stromal proliferation was present at 12 and 24 hours, respectively, and peaked at 7 days and 72 hours, respectively. Some proliferation in the ICRS-implanted group continued through the 6- month follow-up, and myofibroblast-like cells differentiated one to three months after ICRS implantation. The segments rotated within the stroma as the limbal inferior angle approached the epithelium. Conclusions Wound healing after ICRS implantation in hen corneas was similar to that of other corneal surgical wounds in stages. However, there were some specific features related to the small size of the epithelial wound and the device permanently implanted inside the cornea.es
dc.format.mimetypeapplication/pdfes
dc.language.isoenges
dc.publisherSpringer Verlages
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectGallinas - Ensayos técnicoses
dc.subjectCórnea - Cirugía
dc.titleTissue reaction after intrastromal corneal ring implantation in an experimental animal modeles
dc.typeinfo:eu-repo/semantics/articlees
dc.identifier.doi10.1007/s00417-015-2959-5es
dc.identifier.publicationfirstpage1071es
dc.identifier.publicationlastpage1083es
dc.identifier.publicationtitleGraefe's Archive for Clinical and Experimental Ophthalmologyes
dc.identifier.publicationvolume253es
dc.peerreviewedSIes
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International


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